From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme

A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry a...

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Autores principales: Sorrentino, Ilaria, Stanzione, Ilaria, Nedellec, Yannig, Piscitelli, Alessandra, Giardina, Paola, Le Goff, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312733/
https://www.ncbi.nlm.nih.gov/pubmed/32466417
http://dx.doi.org/10.3390/ijms21113741
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author Sorrentino, Ilaria
Stanzione, Ilaria
Nedellec, Yannig
Piscitelli, Alessandra
Giardina, Paola
Le Goff, Alan
author_facet Sorrentino, Ilaria
Stanzione, Ilaria
Nedellec, Yannig
Piscitelli, Alessandra
Giardina, Paola
Le Goff, Alan
author_sort Sorrentino, Ilaria
collection PubMed
description A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry and Raman spectroscopy, and finally employed in the biosensing of phenols such as catechol and dopamine. This strategy paves the way for the functionalization of nanomaterials by hydrophobin domains of chimeric enzymes and their use in a variety of electrochemical applications.
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spelling pubmed-73127332020-06-26 From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme Sorrentino, Ilaria Stanzione, Ilaria Nedellec, Yannig Piscitelli, Alessandra Giardina, Paola Le Goff, Alan Int J Mol Sci Article A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry and Raman spectroscopy, and finally employed in the biosensing of phenols such as catechol and dopamine. This strategy paves the way for the functionalization of nanomaterials by hydrophobin domains of chimeric enzymes and their use in a variety of electrochemical applications. MDPI 2020-05-26 /pmc/articles/PMC7312733/ /pubmed/32466417 http://dx.doi.org/10.3390/ijms21113741 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sorrentino, Ilaria
Stanzione, Ilaria
Nedellec, Yannig
Piscitelli, Alessandra
Giardina, Paola
Le Goff, Alan
From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
title From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
title_full From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
title_fullStr From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
title_full_unstemmed From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
title_short From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
title_sort from graphite to laccase biofunctionalized few-layer graphene: a “one pot” approach using a chimeric enzyme
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312733/
https://www.ncbi.nlm.nih.gov/pubmed/32466417
http://dx.doi.org/10.3390/ijms21113741
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