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Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts

As events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts...

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Autores principales: Nazet, Ute, Grässel, Susanne, Jantsch, Jonathan, Proff, Peter, Schröder, Agnes, Kirschneck, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312748/
https://www.ncbi.nlm.nih.gov/pubmed/32485947
http://dx.doi.org/10.3390/ijms21113874
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author Nazet, Ute
Grässel, Susanne
Jantsch, Jonathan
Proff, Peter
Schröder, Agnes
Kirschneck, Christian
author_facet Nazet, Ute
Grässel, Susanne
Jantsch, Jonathan
Proff, Peter
Schröder, Agnes
Kirschneck, Christian
author_sort Nazet, Ute
collection PubMed
description As events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts. Three distinct models of tensile stretching were applied: static isotropic tensile strain at 0 Hz, 16% tension for 48 h; short-term high-frequency cyclic tension at 1 Hz, 10% tension for 4 h; and dynamic tensile stretching for 48 h, consisting of two blocks of moderate stretching at 0.2 Hz, 2%, advanced stretching at 0.5 Hz, 15%, or a combination of both. General signs of inflammation were present after static isotropic tension, whereas short-term high-frequency cyclic tension showed increased levels of IL-6 paired with diminished levels of IL-1β. Reduced inflammatory effects of TNF-α, IL-6, and IL-1β were observed when exposed to advanced stretching. Long-term tensile strain induced extracellular matrix remodelling at the gene and protein levels. While hyaluronan acid synthesis was increased with static tensile strain, dynamic tensile stretching had a reducing effect. Our study revealed that proinflammatory markers were activated by mechanical strain as seen in static isotropic tension and short-term high-frequency tensile strain, whereas long-term exposure induced extracellular matrix remodelling processes.
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spelling pubmed-73127482020-06-26 Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts Nazet, Ute Grässel, Susanne Jantsch, Jonathan Proff, Peter Schröder, Agnes Kirschneck, Christian Int J Mol Sci Article As events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts. Three distinct models of tensile stretching were applied: static isotropic tensile strain at 0 Hz, 16% tension for 48 h; short-term high-frequency cyclic tension at 1 Hz, 10% tension for 4 h; and dynamic tensile stretching for 48 h, consisting of two blocks of moderate stretching at 0.2 Hz, 2%, advanced stretching at 0.5 Hz, 15%, or a combination of both. General signs of inflammation were present after static isotropic tension, whereas short-term high-frequency cyclic tension showed increased levels of IL-6 paired with diminished levels of IL-1β. Reduced inflammatory effects of TNF-α, IL-6, and IL-1β were observed when exposed to advanced stretching. Long-term tensile strain induced extracellular matrix remodelling at the gene and protein levels. While hyaluronan acid synthesis was increased with static tensile strain, dynamic tensile stretching had a reducing effect. Our study revealed that proinflammatory markers were activated by mechanical strain as seen in static isotropic tension and short-term high-frequency tensile strain, whereas long-term exposure induced extracellular matrix remodelling processes. MDPI 2020-05-29 /pmc/articles/PMC7312748/ /pubmed/32485947 http://dx.doi.org/10.3390/ijms21113874 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nazet, Ute
Grässel, Susanne
Jantsch, Jonathan
Proff, Peter
Schröder, Agnes
Kirschneck, Christian
Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
title Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
title_full Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
title_fullStr Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
title_full_unstemmed Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
title_short Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
title_sort early oa stage like response occurs after dynamic stretching of human synovial fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312748/
https://www.ncbi.nlm.nih.gov/pubmed/32485947
http://dx.doi.org/10.3390/ijms21113874
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