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N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia/reperfusion (I/R) injury in C57BL/6J mice, 8 weeks-old, weighing 25–30 g, with...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313069/ https://www.ncbi.nlm.nih.gov/pubmed/32526845 http://dx.doi.org/10.3390/ijms21114106 |
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author | Chaves Cayuela, Natalie Kiyomi Koike, Marcia Jacysyn, Jacqueline de Fátima Rasslan, Roberto Azevedo Cerqueira, Anderson Romério Pereira Costa, Soraia Katia Picanço Diniz-Júnior, José Antônio Massazo Utiyama, Edivaldo Frasson de Souza Montero, Edna |
author_facet | Chaves Cayuela, Natalie Kiyomi Koike, Marcia Jacysyn, Jacqueline de Fátima Rasslan, Roberto Azevedo Cerqueira, Anderson Romério Pereira Costa, Soraia Katia Picanço Diniz-Júnior, José Antônio Massazo Utiyama, Edivaldo Frasson de Souza Montero, Edna |
author_sort | Chaves Cayuela, Natalie |
collection | PubMed |
description | N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia/reperfusion (I/R) injury in C57BL/6J mice, 8 weeks-old, weighing 25–30 g, with steatohepatitis induced by a methionine- and choline-deficient (MCD) diet. Groups: MCD group (steatohepatitis), MCD-I/R group (steatohepatitis plus 30 min of 70% liver ischemia and 24 h of reperfusion), MCD-I/R+NAC group (same as MCD-I/R group plus 150 mg/kg NAC 15 min before ischemia), and control group (normal AIN-93M diet). Liver enzymes and histopathology; nitrite and TBARS (thiobarbituric acid reactive substances) levels; pro-inflammatory cytokines; antioxidants enzymes; Nrf2 (nuclear factor erythroid-2-related factor 2) expression; and apoptosis were evaluated. In the group treated with NAC, reductions in inflammatory infiltration; AST (aspartate aminotransferase), nitrite, and TBARS levels; GPx (gutathione peroxidase) activity; cytokines synthesis; and number of apoptotic cells were observed while the GR (glutathione reductase) activity was increased. No differences were observed in Nfr2 expression or in SOD (superoxide dismutase), CAT (catalase), and GST (glutathione S-transferase) activities. Thus, it may be concluded that NAC exerts beneficial effects on mice livers with steatohepatitis submitted to I/R by reducing oxidative stress, inflammatory response, and cell death. |
format | Online Article Text |
id | pubmed-7313069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73130692020-06-29 N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice Chaves Cayuela, Natalie Kiyomi Koike, Marcia Jacysyn, Jacqueline de Fátima Rasslan, Roberto Azevedo Cerqueira, Anderson Romério Pereira Costa, Soraia Katia Picanço Diniz-Júnior, José Antônio Massazo Utiyama, Edivaldo Frasson de Souza Montero, Edna Int J Mol Sci Article N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia/reperfusion (I/R) injury in C57BL/6J mice, 8 weeks-old, weighing 25–30 g, with steatohepatitis induced by a methionine- and choline-deficient (MCD) diet. Groups: MCD group (steatohepatitis), MCD-I/R group (steatohepatitis plus 30 min of 70% liver ischemia and 24 h of reperfusion), MCD-I/R+NAC group (same as MCD-I/R group plus 150 mg/kg NAC 15 min before ischemia), and control group (normal AIN-93M diet). Liver enzymes and histopathology; nitrite and TBARS (thiobarbituric acid reactive substances) levels; pro-inflammatory cytokines; antioxidants enzymes; Nrf2 (nuclear factor erythroid-2-related factor 2) expression; and apoptosis were evaluated. In the group treated with NAC, reductions in inflammatory infiltration; AST (aspartate aminotransferase), nitrite, and TBARS levels; GPx (gutathione peroxidase) activity; cytokines synthesis; and number of apoptotic cells were observed while the GR (glutathione reductase) activity was increased. No differences were observed in Nfr2 expression or in SOD (superoxide dismutase), CAT (catalase), and GST (glutathione S-transferase) activities. Thus, it may be concluded that NAC exerts beneficial effects on mice livers with steatohepatitis submitted to I/R by reducing oxidative stress, inflammatory response, and cell death. MDPI 2020-06-09 /pmc/articles/PMC7313069/ /pubmed/32526845 http://dx.doi.org/10.3390/ijms21114106 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chaves Cayuela, Natalie Kiyomi Koike, Marcia Jacysyn, Jacqueline de Fátima Rasslan, Roberto Azevedo Cerqueira, Anderson Romério Pereira Costa, Soraia Katia Picanço Diniz-Júnior, José Antônio Massazo Utiyama, Edivaldo Frasson de Souza Montero, Edna N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice |
title | N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice |
title_full | N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice |
title_fullStr | N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice |
title_full_unstemmed | N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice |
title_short | N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice |
title_sort | n-acetylcysteine reduced ischemia and reperfusion damage associated with steatohepatitis in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313069/ https://www.ncbi.nlm.nih.gov/pubmed/32526845 http://dx.doi.org/10.3390/ijms21114106 |
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