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N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice

N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia/reperfusion (I/R) injury in C57BL/6J mice, 8 weeks-old, weighing 25–30 g, with...

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Autores principales: Chaves Cayuela, Natalie, Kiyomi Koike, Marcia, Jacysyn, Jacqueline de Fátima, Rasslan, Roberto, Azevedo Cerqueira, Anderson Romério, Pereira Costa, Soraia Katia, Picanço Diniz-Júnior, José Antônio, Massazo Utiyama, Edivaldo, Frasson de Souza Montero, Edna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313069/
https://www.ncbi.nlm.nih.gov/pubmed/32526845
http://dx.doi.org/10.3390/ijms21114106
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author Chaves Cayuela, Natalie
Kiyomi Koike, Marcia
Jacysyn, Jacqueline de Fátima
Rasslan, Roberto
Azevedo Cerqueira, Anderson Romério
Pereira Costa, Soraia Katia
Picanço Diniz-Júnior, José Antônio
Massazo Utiyama, Edivaldo
Frasson de Souza Montero, Edna
author_facet Chaves Cayuela, Natalie
Kiyomi Koike, Marcia
Jacysyn, Jacqueline de Fátima
Rasslan, Roberto
Azevedo Cerqueira, Anderson Romério
Pereira Costa, Soraia Katia
Picanço Diniz-Júnior, José Antônio
Massazo Utiyama, Edivaldo
Frasson de Souza Montero, Edna
author_sort Chaves Cayuela, Natalie
collection PubMed
description N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia/reperfusion (I/R) injury in C57BL/6J mice, 8 weeks-old, weighing 25–30 g, with steatohepatitis induced by a methionine- and choline-deficient (MCD) diet. Groups: MCD group (steatohepatitis), MCD-I/R group (steatohepatitis plus 30 min of 70% liver ischemia and 24 h of reperfusion), MCD-I/R+NAC group (same as MCD-I/R group plus 150 mg/kg NAC 15 min before ischemia), and control group (normal AIN-93M diet). Liver enzymes and histopathology; nitrite and TBARS (thiobarbituric acid reactive substances) levels; pro-inflammatory cytokines; antioxidants enzymes; Nrf2 (nuclear factor erythroid-2-related factor 2) expression; and apoptosis were evaluated. In the group treated with NAC, reductions in inflammatory infiltration; AST (aspartate aminotransferase), nitrite, and TBARS levels; GPx (gutathione peroxidase) activity; cytokines synthesis; and number of apoptotic cells were observed while the GR (glutathione reductase) activity was increased. No differences were observed in Nfr2 expression or in SOD (superoxide dismutase), CAT (catalase), and GST (glutathione S-transferase) activities. Thus, it may be concluded that NAC exerts beneficial effects on mice livers with steatohepatitis submitted to I/R by reducing oxidative stress, inflammatory response, and cell death.
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spelling pubmed-73130692020-06-29 N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice Chaves Cayuela, Natalie Kiyomi Koike, Marcia Jacysyn, Jacqueline de Fátima Rasslan, Roberto Azevedo Cerqueira, Anderson Romério Pereira Costa, Soraia Katia Picanço Diniz-Júnior, José Antônio Massazo Utiyama, Edivaldo Frasson de Souza Montero, Edna Int J Mol Sci Article N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia/reperfusion (I/R) injury in C57BL/6J mice, 8 weeks-old, weighing 25–30 g, with steatohepatitis induced by a methionine- and choline-deficient (MCD) diet. Groups: MCD group (steatohepatitis), MCD-I/R group (steatohepatitis plus 30 min of 70% liver ischemia and 24 h of reperfusion), MCD-I/R+NAC group (same as MCD-I/R group plus 150 mg/kg NAC 15 min before ischemia), and control group (normal AIN-93M diet). Liver enzymes and histopathology; nitrite and TBARS (thiobarbituric acid reactive substances) levels; pro-inflammatory cytokines; antioxidants enzymes; Nrf2 (nuclear factor erythroid-2-related factor 2) expression; and apoptosis were evaluated. In the group treated with NAC, reductions in inflammatory infiltration; AST (aspartate aminotransferase), nitrite, and TBARS levels; GPx (gutathione peroxidase) activity; cytokines synthesis; and number of apoptotic cells were observed while the GR (glutathione reductase) activity was increased. No differences were observed in Nfr2 expression or in SOD (superoxide dismutase), CAT (catalase), and GST (glutathione S-transferase) activities. Thus, it may be concluded that NAC exerts beneficial effects on mice livers with steatohepatitis submitted to I/R by reducing oxidative stress, inflammatory response, and cell death. MDPI 2020-06-09 /pmc/articles/PMC7313069/ /pubmed/32526845 http://dx.doi.org/10.3390/ijms21114106 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chaves Cayuela, Natalie
Kiyomi Koike, Marcia
Jacysyn, Jacqueline de Fátima
Rasslan, Roberto
Azevedo Cerqueira, Anderson Romério
Pereira Costa, Soraia Katia
Picanço Diniz-Júnior, José Antônio
Massazo Utiyama, Edivaldo
Frasson de Souza Montero, Edna
N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
title N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
title_full N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
title_fullStr N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
title_full_unstemmed N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
title_short N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
title_sort n-acetylcysteine reduced ischemia and reperfusion damage associated with steatohepatitis in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313069/
https://www.ncbi.nlm.nih.gov/pubmed/32526845
http://dx.doi.org/10.3390/ijms21114106
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