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An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

BACKGROUND: A neutral, heat-sensitive serine protease (NHSSP) originating from the feather-degrading fungus Onygena corvina (O. corvina) was described and defined as an alkaline serine protease of the subtilisin type S8 family, exhibiting an enzymatic activity at neutral pH. Generally, broad specifi...

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Detalles Bibliográficos
Autores principales: Skowron, Piotr M., Krefft, Daria, Brodzik, Robert, Kasperkiewicz, Paulina, Drag, Marcin, Koller, Klaus-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313183/
https://www.ncbi.nlm.nih.gov/pubmed/32580707
http://dx.doi.org/10.1186/s12934-020-01392-3