MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation

The tumor suppressive role of MIR22HG has been studied in several types of cancer. We analyzed the TCGA dataset and found the down-regulation of MIR22HG in bladder cancer (BC). Bioinformatics analysis predicted the interaction between MIR22HG and miR-486. The direct interaction between MIR22HG and m...

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Detalles Bibliográficos
Autores principales: Tang, Qisheng, Jiang, Xue, Ma, Shanjin, Wang, Lei, Li, Ruixiao, Ma, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Cancer
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313441/
https://www.ncbi.nlm.nih.gov/pubmed/32500915
http://dx.doi.org/10.1042/BSR20193991
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author Tang, Qisheng
Jiang, Xue
Ma, Shanjin
Wang, Lei
Li, Ruixiao
Ma, Jianjun
author_facet Tang, Qisheng
Jiang, Xue
Ma, Shanjin
Wang, Lei
Li, Ruixiao
Ma, Jianjun
author_sort Tang, Qisheng
collection PubMed
description The tumor suppressive role of MIR22HG has been studied in several types of cancer. We analyzed the TCGA dataset and found the down-regulation of MIR22HG in bladder cancer (BC). Bioinformatics analysis predicted the interaction between MIR22HG and miR-486. The direct interaction between MIR22HG and miR-486 was also confirmed by dual luciferase assay. However, overexpression of these two factors did not significantly affect the expression of each other. Interestingly, overexpression of MIR22HG led to up-regulated phosphatase and tensin homolog (PTEN), which is a target of miR-486. In cell proliferation assay, overexpression of MIR22HG and PTEN led to decreased rates of BC cell proliferation. Moreover, overexpression of miR-486 played an opposite role and attenuated the effects of overexpression of MIR22HG and PTEN. Therefore, MIR22HG regulates miR-486/PTEN axis to promote cell proliferation in BC.
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spelling pubmed-73134412020-06-26 MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation Tang, Qisheng Jiang, Xue Ma, Shanjin Wang, Lei Li, Ruixiao Ma, Jianjun Biosci Rep Cancer The tumor suppressive role of MIR22HG has been studied in several types of cancer. We analyzed the TCGA dataset and found the down-regulation of MIR22HG in bladder cancer (BC). Bioinformatics analysis predicted the interaction between MIR22HG and miR-486. The direct interaction between MIR22HG and miR-486 was also confirmed by dual luciferase assay. However, overexpression of these two factors did not significantly affect the expression of each other. Interestingly, overexpression of MIR22HG led to up-regulated phosphatase and tensin homolog (PTEN), which is a target of miR-486. In cell proliferation assay, overexpression of MIR22HG and PTEN led to decreased rates of BC cell proliferation. Moreover, overexpression of miR-486 played an opposite role and attenuated the effects of overexpression of MIR22HG and PTEN. Therefore, MIR22HG regulates miR-486/PTEN axis to promote cell proliferation in BC. Portland Press Ltd. 2020-06-23 /pmc/articles/PMC7313441/ /pubmed/32500915 http://dx.doi.org/10.1042/BSR20193991 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Cancer
Tang, Qisheng
Jiang, Xue
Ma, Shanjin
Wang, Lei
Li, Ruixiao
Ma, Jianjun
MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation
title MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation
title_full MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation
title_fullStr MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation
title_full_unstemmed MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation
title_short MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation
title_sort mir22hg regulates mir-486/pten axis in bladder cancer to promote cell proliferation
topic Cancer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313441/
https://www.ncbi.nlm.nih.gov/pubmed/32500915
http://dx.doi.org/10.1042/BSR20193991
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AT jiangxue mir22hgregulatesmir486ptenaxisinbladdercancertopromotecellproliferation
AT mashanjin mir22hgregulatesmir486ptenaxisinbladdercancertopromotecellproliferation
AT wanglei mir22hgregulatesmir486ptenaxisinbladdercancertopromotecellproliferation
AT liruixiao mir22hgregulatesmir486ptenaxisinbladdercancertopromotecellproliferation
AT majianjun mir22hgregulatesmir486ptenaxisinbladdercancertopromotecellproliferation

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