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OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)

The chaperonin 60 (Cpn60) protein is of great importance to plants due to its involvement in modulating the folding of numerous chloroplast protein polypeptides. In chloroplasts, Cpn60 is differentiated into two subunit types—Cpn60α and Cpn60β and the rice genome encodes three α and three β plastid...

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Autores principales: Wu, Qingfei, Zhang, Cheng, Chen, Yue, Zhou, Kaiyue, Zhan, Yihua, Jiang, Dean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313468/
https://www.ncbi.nlm.nih.gov/pubmed/32512821
http://dx.doi.org/10.3390/ijms21114023
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author Wu, Qingfei
Zhang, Cheng
Chen, Yue
Zhou, Kaiyue
Zhan, Yihua
Jiang, Dean
author_facet Wu, Qingfei
Zhang, Cheng
Chen, Yue
Zhou, Kaiyue
Zhan, Yihua
Jiang, Dean
author_sort Wu, Qingfei
collection PubMed
description The chaperonin 60 (Cpn60) protein is of great importance to plants due to its involvement in modulating the folding of numerous chloroplast protein polypeptides. In chloroplasts, Cpn60 is differentiated into two subunit types—Cpn60α and Cpn60β and the rice genome encodes three α and three β plastid chaperonin subunits. However, the functions of Cpn60 family members in rice were poorly understood. In order to investigate the molecular mechanism of OsCpn60β1, we attempted to disrupt the OsCpn60β1 gene by CRISPR/Cas9-mediated targeted mutagenesis in this study. We succeeded in the production of homozygous OsCpn60β1 knockout rice plants. The OsCpn60β1 mutant displayed a striking albino leaf phenotype and was seedling lethal. Electron microscopy observation demonstrated that chloroplasts were severely disrupted in the OsCpn60β1 mutant. In addition, OsCpn60β1 was located in the chloroplast and OsCpn60β1 is constitutively expressed in various tissues particularly in the green tissues. The label-free qualitative proteomics showed that photosynthesis-related pathways and ribosomal pathways were significantly inhibited in OsCpn60β1 mutants. These results indicate that OsCpn60β1 is essential for chloroplast development in rice.
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spelling pubmed-73134682020-06-29 OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.) Wu, Qingfei Zhang, Cheng Chen, Yue Zhou, Kaiyue Zhan, Yihua Jiang, Dean Int J Mol Sci Article The chaperonin 60 (Cpn60) protein is of great importance to plants due to its involvement in modulating the folding of numerous chloroplast protein polypeptides. In chloroplasts, Cpn60 is differentiated into two subunit types—Cpn60α and Cpn60β and the rice genome encodes three α and three β plastid chaperonin subunits. However, the functions of Cpn60 family members in rice were poorly understood. In order to investigate the molecular mechanism of OsCpn60β1, we attempted to disrupt the OsCpn60β1 gene by CRISPR/Cas9-mediated targeted mutagenesis in this study. We succeeded in the production of homozygous OsCpn60β1 knockout rice plants. The OsCpn60β1 mutant displayed a striking albino leaf phenotype and was seedling lethal. Electron microscopy observation demonstrated that chloroplasts were severely disrupted in the OsCpn60β1 mutant. In addition, OsCpn60β1 was located in the chloroplast and OsCpn60β1 is constitutively expressed in various tissues particularly in the green tissues. The label-free qualitative proteomics showed that photosynthesis-related pathways and ribosomal pathways were significantly inhibited in OsCpn60β1 mutants. These results indicate that OsCpn60β1 is essential for chloroplast development in rice. MDPI 2020-06-04 /pmc/articles/PMC7313468/ /pubmed/32512821 http://dx.doi.org/10.3390/ijms21114023 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Qingfei
Zhang, Cheng
Chen, Yue
Zhou, Kaiyue
Zhan, Yihua
Jiang, Dean
OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)
title OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)
title_full OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)
title_fullStr OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)
title_full_unstemmed OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)
title_short OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)
title_sort oscpn60β1 is essential for chloroplast development in rice (oryza sativa l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313468/
https://www.ncbi.nlm.nih.gov/pubmed/32512821
http://dx.doi.org/10.3390/ijms21114023
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