Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allerg...

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Autores principales: Bastiaan‐Net, Shanna, Pina‐Pérez, Maria C., Dekkers, Bas J. W., Westphal, Adrie H., America, Antoine H. P., Ariëns, Renata M. C., de Jong, Nicolette W., Wichers, Harry J., Mes, Jurriaan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Full‐length Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314402/
https://www.ncbi.nlm.nih.gov/pubmed/32219913
http://dx.doi.org/10.1002/pro.3856
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author Bastiaan‐Net, Shanna
Pina‐Pérez, Maria C.
Dekkers, Bas J. W.
Westphal, Adrie H.
America, Antoine H. P.
Ariëns, Renata M. C.
de Jong, Nicolette W.
Wichers, Harry J.
Mes, Jurriaan J.
author_facet Bastiaan‐Net, Shanna
Pina‐Pérez, Maria C.
Dekkers, Bas J. W.
Westphal, Adrie H.
America, Antoine H. P.
Ariëns, Renata M. C.
de Jong, Nicolette W.
Wichers, Harry J.
Mes, Jurriaan J.
author_sort Bastiaan‐Net, Shanna
collection PubMed
description Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA‐seq analysis, we were able to identify several PR10‐like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.
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spelling pubmed-73144022020-06-25 Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut Bastiaan‐Net, Shanna Pina‐Pérez, Maria C. Dekkers, Bas J. W. Westphal, Adrie H. America, Antoine H. P. Ariëns, Renata M. C. de Jong, Nicolette W. Wichers, Harry J. Mes, Jurriaan J. Protein Sci Full‐length Papers Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA‐seq analysis, we were able to identify several PR10‐like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens. John Wiley & Sons, Inc. 2020-05-16 2020-07 /pmc/articles/PMC7314402/ /pubmed/32219913 http://dx.doi.org/10.1002/pro.3856 Text en © 2020 The Authors. Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full‐length Papers
Bastiaan‐Net, Shanna
Pina‐Pérez, Maria C.
Dekkers, Bas J. W.
Westphal, Adrie H.
America, Antoine H. P.
Ariëns, Renata M. C.
de Jong, Nicolette W.
Wichers, Harry J.
Mes, Jurriaan J.
Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
title Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
title_full Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
title_fullStr Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
title_full_unstemmed Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
title_short Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
title_sort identification and in silico bioinformatics analysis of pr10 proteins in cashew nut
topic Full‐length Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314402/
https://www.ncbi.nlm.nih.gov/pubmed/32219913
http://dx.doi.org/10.1002/pro.3856
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