Cargando…
Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing
Preparations from Arabian Gulf catfish (Arius bilineatus, Val) epidermal gel secretion (PCEGS) effectively heal chronic wounds in diabetic patients. However, specific lipid components of PCEGS that are responsible for various aspects of wound healing are unknown. Here, we report for the first time t...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314935/ https://www.ncbi.nlm.nih.gov/pubmed/32625093 http://dx.doi.org/10.3389/fphar.2020.00899 |
_version_ | 1783550157789331456 |
---|---|
author | Al-Hassan, Jassim M. Hinek, Aleksander Renno, Waleed M. Wang, Yanting Liu, Yuan Fang Guan, Rui Wen, Xiao-Yen Litvack, Michael L. Lindenmaier, Andras Afzal, Mohammad Paul, Bincy Oommen, Sosamma Nair, Divya Kumar, Jijin Khan, Meraj A. Palaniyar, Nades Pace-Asciak, Cecil |
author_facet | Al-Hassan, Jassim M. Hinek, Aleksander Renno, Waleed M. Wang, Yanting Liu, Yuan Fang Guan, Rui Wen, Xiao-Yen Litvack, Michael L. Lindenmaier, Andras Afzal, Mohammad Paul, Bincy Oommen, Sosamma Nair, Divya Kumar, Jijin Khan, Meraj A. Palaniyar, Nades Pace-Asciak, Cecil |
author_sort | Al-Hassan, Jassim M. |
collection | PubMed |
description | Preparations from Arabian Gulf catfish (Arius bilineatus, Val) epidermal gel secretion (PCEGS) effectively heal chronic wounds in diabetic patients. However, specific lipid components of PCEGS that are responsible for various aspects of wound healing are unknown. Here, we report for the first time that, i) a unique preparation containing only proteins and lipids (Fraction B, FB), derived from the PCEGS accelerated the healing of experimental dermal wounds in female rats (transdermal punch biopsy) in vivo. Histological analyses showed that topical treatment of these wounds with FB promoted the migration of fibroblasts, facilitated the production of extracellular matrix (collagen, fibronectin), induced capillary formation and recruitment of immune cells, and accelerated overall wound healing by day 4 (tested at 1, 2, 3, 4, and 10 days; n=15 for vehicle; n=15 for FB treatment), ii) the lipids responsible for different stages of wound healing were separated into a protein-free bioactive lipid fraction, Ft, which contained a few common long-chain fatty acids, a unique furan fatty acid (F6) and a cholesterol metabolite, cholesta-3,5-diene (S5). Ft (the partially purified lipid fraction of PCEGS), and F6 and S5 present in Ft, proved to be bioactive for wound healing in human dermal fibroblasts. Ft increased the production and extracellular deposition of collagen and fibronectin, ex vivo, iii) Ft and its subcomponents, pure F6 and S5, also promoted human dermal fibroblast migration into the scratch wound gaps, ex vivo, iv) Ft, F6, and S5 promoted the recruitment of neutrophils (Green fluorescence protein labeled) to the site of injury in the transected tailfins of transgenic zebrafish, in vivo, v) Ft, but not F6 or S5, promoted the regeneration of tissues at the wound site in the transgenic zebrafish tailfin, in vivo. Therefore, we conclude that lipid fraction Ft from PCEGS contains the components necessary to promote complete wound healing, and F6 and S5 are responsible for promoting fibroblast and neutrophil recruitment to the site of wounds. |
format | Online Article Text |
id | pubmed-7314935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73149352020-07-02 Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing Al-Hassan, Jassim M. Hinek, Aleksander Renno, Waleed M. Wang, Yanting Liu, Yuan Fang Guan, Rui Wen, Xiao-Yen Litvack, Michael L. Lindenmaier, Andras Afzal, Mohammad Paul, Bincy Oommen, Sosamma Nair, Divya Kumar, Jijin Khan, Meraj A. Palaniyar, Nades Pace-Asciak, Cecil Front Pharmacol Pharmacology Preparations from Arabian Gulf catfish (Arius bilineatus, Val) epidermal gel secretion (PCEGS) effectively heal chronic wounds in diabetic patients. However, specific lipid components of PCEGS that are responsible for various aspects of wound healing are unknown. Here, we report for the first time that, i) a unique preparation containing only proteins and lipids (Fraction B, FB), derived from the PCEGS accelerated the healing of experimental dermal wounds in female rats (transdermal punch biopsy) in vivo. Histological analyses showed that topical treatment of these wounds with FB promoted the migration of fibroblasts, facilitated the production of extracellular matrix (collagen, fibronectin), induced capillary formation and recruitment of immune cells, and accelerated overall wound healing by day 4 (tested at 1, 2, 3, 4, and 10 days; n=15 for vehicle; n=15 for FB treatment), ii) the lipids responsible for different stages of wound healing were separated into a protein-free bioactive lipid fraction, Ft, which contained a few common long-chain fatty acids, a unique furan fatty acid (F6) and a cholesterol metabolite, cholesta-3,5-diene (S5). Ft (the partially purified lipid fraction of PCEGS), and F6 and S5 present in Ft, proved to be bioactive for wound healing in human dermal fibroblasts. Ft increased the production and extracellular deposition of collagen and fibronectin, ex vivo, iii) Ft and its subcomponents, pure F6 and S5, also promoted human dermal fibroblast migration into the scratch wound gaps, ex vivo, iv) Ft, F6, and S5 promoted the recruitment of neutrophils (Green fluorescence protein labeled) to the site of injury in the transected tailfins of transgenic zebrafish, in vivo, v) Ft, but not F6 or S5, promoted the regeneration of tissues at the wound site in the transgenic zebrafish tailfin, in vivo. Therefore, we conclude that lipid fraction Ft from PCEGS contains the components necessary to promote complete wound healing, and F6 and S5 are responsible for promoting fibroblast and neutrophil recruitment to the site of wounds. Frontiers Media S.A. 2020-06-18 /pmc/articles/PMC7314935/ /pubmed/32625093 http://dx.doi.org/10.3389/fphar.2020.00899 Text en Copyright © 2020 Al-Hassan, Hinek, Renno, Wang, Liu, Guan, Wen, Litvack, Lindenmaier, Afzal, Paul, Oommen, Nair, Kumar, Khan, Palaniyar and Pace-Asciak http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Al-Hassan, Jassim M. Hinek, Aleksander Renno, Waleed M. Wang, Yanting Liu, Yuan Fang Guan, Rui Wen, Xiao-Yen Litvack, Michael L. Lindenmaier, Andras Afzal, Mohammad Paul, Bincy Oommen, Sosamma Nair, Divya Kumar, Jijin Khan, Meraj A. Palaniyar, Nades Pace-Asciak, Cecil Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing |
title | Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing |
title_full | Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing |
title_fullStr | Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing |
title_full_unstemmed | Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing |
title_short | Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing |
title_sort | potential mechanism of dermal wound treatment with preparations from the skin gel of arabian gulf catfish: a unique furan fatty acid (f6) and cholesta-3,5-diene (s5) recruit neutrophils and fibroblasts to promote wound healing |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314935/ https://www.ncbi.nlm.nih.gov/pubmed/32625093 http://dx.doi.org/10.3389/fphar.2020.00899 |
work_keys_str_mv | AT alhassanjassimm potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT hinekaleksander potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT rennowaleedm potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT wangyanting potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT liuyuanfang potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT guanrui potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT wenxiaoyen potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT litvackmichaell potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT lindenmaierandras potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT afzalmohammad potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT paulbincy potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT oommensosamma potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT nairdivya potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT kumarjijin potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT khanmeraja potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT palaniyarnades potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing AT paceasciakcecil potentialmechanismofdermalwoundtreatmentwithpreparationsfromtheskingelofarabiangulfcatfishauniquefuranfattyacidf6andcholesta35dienes5recruitneutrophilsandfibroblaststopromotewoundhealing |