Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification

This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of t...

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Detalles Bibliográficos
Autores principales: Kitagawa, Masae, Nagamine, Kentaro, Oka, Hiroko, Ouhara, Kazuhisa, Ogawa, Ikuko, Komatsuzawa, Hitoshi, Kurihara, Hidemi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315161/
https://www.ncbi.nlm.nih.gov/pubmed/32592714
http://dx.doi.org/10.1016/j.ab.2020.113812
Descripción
Sumario:This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnm-positive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples.

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