Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification

This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of t...

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Autores principales: Kitagawa, Masae, Nagamine, Kentaro, Oka, Hiroko, Ouhara, Kazuhisa, Ogawa, Ikuko, Komatsuzawa, Hitoshi, Kurihara, Hidemi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315161/
https://www.ncbi.nlm.nih.gov/pubmed/32592714
http://dx.doi.org/10.1016/j.ab.2020.113812
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author Kitagawa, Masae
Nagamine, Kentaro
Oka, Hiroko
Ouhara, Kazuhisa
Ogawa, Ikuko
Komatsuzawa, Hitoshi
Kurihara, Hidemi
author_facet Kitagawa, Masae
Nagamine, Kentaro
Oka, Hiroko
Ouhara, Kazuhisa
Ogawa, Ikuko
Komatsuzawa, Hitoshi
Kurihara, Hidemi
author_sort Kitagawa, Masae
collection PubMed
description This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnm-positive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples.
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spelling pubmed-73151612020-06-25 Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification Kitagawa, Masae Nagamine, Kentaro Oka, Hiroko Ouhara, Kazuhisa Ogawa, Ikuko Komatsuzawa, Hitoshi Kurihara, Hidemi Anal Biochem Article This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnm-positive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples. Elsevier Inc. 2020-09-15 2020-06-25 /pmc/articles/PMC7315161/ /pubmed/32592714 http://dx.doi.org/10.1016/j.ab.2020.113812 Text en © 2020 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Kitagawa, Masae
Nagamine, Kentaro
Oka, Hiroko
Ouhara, Kazuhisa
Ogawa, Ikuko
Komatsuzawa, Hitoshi
Kurihara, Hidemi
Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
title Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
title_full Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
title_fullStr Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
title_full_unstemmed Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
title_short Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
title_sort rapid detection of the streptococcus mutans cnm gene by loop-mediated isothermal amplification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315161/
https://www.ncbi.nlm.nih.gov/pubmed/32592714
http://dx.doi.org/10.1016/j.ab.2020.113812
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