Cargando…
Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”
Acidobacteria represents one of the most dominant bacterial groups across diverse ecosystems. However, insight into their ecology and physiology has been hampered by difficulties in cultivating members of this phylum. Previous cultivation efforts have suggested an important role of trace elements fo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315648/ https://www.ncbi.nlm.nih.gov/pubmed/32625179 http://dx.doi.org/10.3389/fmicb.2020.01227 |
_version_ | 1783550299004207104 |
---|---|
author | Costa, Ohana Y. A. Oguejiofor, Chidinma Zühlke, Daniela Barreto, Cristine C. Wünsche, Christine Riedel, Katharina Kuramae, Eiko E. |
author_facet | Costa, Ohana Y. A. Oguejiofor, Chidinma Zühlke, Daniela Barreto, Cristine C. Wünsche, Christine Riedel, Katharina Kuramae, Eiko E. |
author_sort | Costa, Ohana Y. A. |
collection | PubMed |
description | Acidobacteria represents one of the most dominant bacterial groups across diverse ecosystems. However, insight into their ecology and physiology has been hampered by difficulties in cultivating members of this phylum. Previous cultivation efforts have suggested an important role of trace elements for the proliferation of Acidobacteria, however, the impact of these metals on their growth and metabolism is not known. In order to gain insight into this relationship, we evaluated the effect of trace element solution SL10 on the growth of two strains (5B5 and WH15) of Acidobacteria belonging to the genus Granulicella and studied the proteomic responses to manganese (Mn). Granulicella species had highest growth with the addition of Mn, as well as higher tolerance to this metal compared to seven other metal salts. Variations in tolerance to metal salt concentrations suggests that Granulicella sp. strains possess different mechanisms to deal with metal ion homeostasis and stress. Furthermore, Granulicella sp. 5B5 might be more adapted to survive in an environment with higher concentration of several metal ions when compared to Granulicella sp. WH15. The proteomic profiles of both strains indicated that Mn was more important in enhancing enzymatic activity than to protein expression regulation. In the genomic analyses, we did not find the most common transcriptional regulation of Mn homeostasis, but we found candidate transporters that could be potentially involved in Mn homeostasis for Granulicella species. The presence of such transporters might be involved in tolerance to higher Mn concentrations, improving the adaptability of bacteria to metal enriched environments, such as the decaying wood-rich Mn environment from which these two Granulicella strains were isolated. |
format | Online Article Text |
id | pubmed-7315648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73156482020-07-02 Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia” Costa, Ohana Y. A. Oguejiofor, Chidinma Zühlke, Daniela Barreto, Cristine C. Wünsche, Christine Riedel, Katharina Kuramae, Eiko E. Front Microbiol Microbiology Acidobacteria represents one of the most dominant bacterial groups across diverse ecosystems. However, insight into their ecology and physiology has been hampered by difficulties in cultivating members of this phylum. Previous cultivation efforts have suggested an important role of trace elements for the proliferation of Acidobacteria, however, the impact of these metals on their growth and metabolism is not known. In order to gain insight into this relationship, we evaluated the effect of trace element solution SL10 on the growth of two strains (5B5 and WH15) of Acidobacteria belonging to the genus Granulicella and studied the proteomic responses to manganese (Mn). Granulicella species had highest growth with the addition of Mn, as well as higher tolerance to this metal compared to seven other metal salts. Variations in tolerance to metal salt concentrations suggests that Granulicella sp. strains possess different mechanisms to deal with metal ion homeostasis and stress. Furthermore, Granulicella sp. 5B5 might be more adapted to survive in an environment with higher concentration of several metal ions when compared to Granulicella sp. WH15. The proteomic profiles of both strains indicated that Mn was more important in enhancing enzymatic activity than to protein expression regulation. In the genomic analyses, we did not find the most common transcriptional regulation of Mn homeostasis, but we found candidate transporters that could be potentially involved in Mn homeostasis for Granulicella species. The presence of such transporters might be involved in tolerance to higher Mn concentrations, improving the adaptability of bacteria to metal enriched environments, such as the decaying wood-rich Mn environment from which these two Granulicella strains were isolated. Frontiers Media S.A. 2020-06-18 /pmc/articles/PMC7315648/ /pubmed/32625179 http://dx.doi.org/10.3389/fmicb.2020.01227 Text en Copyright © 2020 Costa, Oguejiofor, Zühlke, Barreto, Wünsche, Riedel and Kuramae. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Costa, Ohana Y. A. Oguejiofor, Chidinma Zühlke, Daniela Barreto, Cristine C. Wünsche, Christine Riedel, Katharina Kuramae, Eiko E. Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia” |
title | Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia” |
title_full | Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia” |
title_fullStr | Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia” |
title_full_unstemmed | Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia” |
title_short | Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia” |
title_sort | impact of different trace elements on the growth and proteome of two strains of granulicella, class “acidobacteriia” |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315648/ https://www.ncbi.nlm.nih.gov/pubmed/32625179 http://dx.doi.org/10.3389/fmicb.2020.01227 |
work_keys_str_mv | AT costaohanaya impactofdifferenttraceelementsonthegrowthandproteomeoftwostrainsofgranulicellaclassacidobacteriia AT oguejioforchidinma impactofdifferenttraceelementsonthegrowthandproteomeoftwostrainsofgranulicellaclassacidobacteriia AT zuhlkedaniela impactofdifferenttraceelementsonthegrowthandproteomeoftwostrainsofgranulicellaclassacidobacteriia AT barretocristinec impactofdifferenttraceelementsonthegrowthandproteomeoftwostrainsofgranulicellaclassacidobacteriia AT wunschechristine impactofdifferenttraceelementsonthegrowthandproteomeoftwostrainsofgranulicellaclassacidobacteriia AT riedelkatharina impactofdifferenttraceelementsonthegrowthandproteomeoftwostrainsofgranulicellaclassacidobacteriia AT kuramaeeikoe impactofdifferenttraceelementsonthegrowthandproteomeoftwostrainsofgranulicellaclassacidobacteriia |