Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020

Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnos...

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Detalles Bibliográficos
Autores principales: Muenchhoff, Maximilian, Mairhofer, Helga, Nitschko, Hans, Grzimek-Koschewa, Natascha, Hoffmann, Dieter, Berger, Annemarie, Rabenau, Holger, Widera, Marek, Ackermann, Nikolaus, Konrad, Regina, Zange, Sabine, Graf, Alexander, Krebs, Stefan, Blum, Helmut, Sing, Andreas, Liebl, Bernhard, Wölfel, Roman, Ciesek, Sandra, Drosten, Christian, Protzer, Ulrike, Boehm, Stephan, Keppler, Oliver T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315722/
https://www.ncbi.nlm.nih.gov/pubmed/32583765
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.24.2001057
Descripción
Sumario:Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.

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