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Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020
Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnos...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
European Centre for Disease Prevention and Control (ECDC)
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315722/ https://www.ncbi.nlm.nih.gov/pubmed/32583765 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.24.2001057 |
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| author | Muenchhoff, Maximilian Mairhofer, Helga Nitschko, Hans Grzimek-Koschewa, Natascha Hoffmann, Dieter Berger, Annemarie Rabenau, Holger Widera, Marek Ackermann, Nikolaus Konrad, Regina Zange, Sabine Graf, Alexander Krebs, Stefan Blum, Helmut Sing, Andreas Liebl, Bernhard Wölfel, Roman Ciesek, Sandra Drosten, Christian Protzer, Ulrike Boehm, Stephan Keppler, Oliver T |
| author_facet | Muenchhoff, Maximilian Mairhofer, Helga Nitschko, Hans Grzimek-Koschewa, Natascha Hoffmann, Dieter Berger, Annemarie Rabenau, Holger Widera, Marek Ackermann, Nikolaus Konrad, Regina Zange, Sabine Graf, Alexander Krebs, Stefan Blum, Helmut Sing, Andreas Liebl, Bernhard Wölfel, Roman Ciesek, Sandra Drosten, Christian Protzer, Ulrike Boehm, Stephan Keppler, Oliver T |
| author_sort | Muenchhoff, Maximilian |
| collection | PubMed |
| description | Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic. |
| format | Online Article Text |
| id | pubmed-7315722 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | European Centre for Disease Prevention and Control (ECDC) |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-73157222020-06-29 Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 Muenchhoff, Maximilian Mairhofer, Helga Nitschko, Hans Grzimek-Koschewa, Natascha Hoffmann, Dieter Berger, Annemarie Rabenau, Holger Widera, Marek Ackermann, Nikolaus Konrad, Regina Zange, Sabine Graf, Alexander Krebs, Stefan Blum, Helmut Sing, Andreas Liebl, Bernhard Wölfel, Roman Ciesek, Sandra Drosten, Christian Protzer, Ulrike Boehm, Stephan Keppler, Oliver T Euro Surveill Rapid Communication Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic. European Centre for Disease Prevention and Control (ECDC) 2020-06-18 /pmc/articles/PMC7315722/ /pubmed/32583765 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.24.2001057 Text en This article is copyright of the authors or their affiliated institutions, 2020. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made. |
| spellingShingle | Rapid Communication Muenchhoff, Maximilian Mairhofer, Helga Nitschko, Hans Grzimek-Koschewa, Natascha Hoffmann, Dieter Berger, Annemarie Rabenau, Holger Widera, Marek Ackermann, Nikolaus Konrad, Regina Zange, Sabine Graf, Alexander Krebs, Stefan Blum, Helmut Sing, Andreas Liebl, Bernhard Wölfel, Roman Ciesek, Sandra Drosten, Christian Protzer, Ulrike Boehm, Stephan Keppler, Oliver T Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 |
| title | Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 |
| title_full | Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 |
| title_fullStr | Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 |
| title_full_unstemmed | Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 |
| title_short | Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 |
| title_sort | multicentre comparison of quantitative pcr-based assays to detect sars-cov-2, germany, march 2020 |
| topic | Rapid Communication |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315722/ https://www.ncbi.nlm.nih.gov/pubmed/32583765 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.24.2001057 |
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