Cargando…
Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
The prevalence and zoonotic potential of Cryptosporidium in donkeys is poorly understood. Here, 680 fecal specimens were collected from 178 free-ranging and 502 farmed donkeys in Xinjiang, China. Cryptosporidium was identified using PCR amplification of the small subunit of ribosomal DNA. Cryptospor...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315819/ https://www.ncbi.nlm.nih.gov/pubmed/32584252 http://dx.doi.org/10.1051/parasite/2020042 |
_version_ | 1783550326218948608 |
---|---|
author | Wang, Wen Zhang, Zhenjie Zhang, Ying Zhao, Aiyun Jing, Bo Zhang, Longxian Liu, Pengtao Qi, Meng Zhao, Wei |
author_facet | Wang, Wen Zhang, Zhenjie Zhang, Ying Zhao, Aiyun Jing, Bo Zhang, Longxian Liu, Pengtao Qi, Meng Zhao, Wei |
author_sort | Wang, Wen |
collection | PubMed |
description | The prevalence and zoonotic potential of Cryptosporidium in donkeys is poorly understood. Here, 680 fecal specimens were collected from 178 free-ranging and 502 farmed donkeys in Xinjiang, China. Cryptosporidium was identified using PCR amplification of the small subunit of ribosomal DNA. Cryptosporidium-positive isolates were subtyped using PCR analysis of the 60 kDa glycoprotein gene (gp60). The overall prevalence of Cryptosporidium was 2.4% (16/680), with 3.2% (16/502) in farmed donkeys and 0% (0/178) in free-ranging donkeys. Cryptosporidium hominis (n = 13), C. parvum (n = 1) and Cryptosporidium horse genotype (n = 2) were identified. The C. hominis isolates belonged to two subtypes, IkA16 (n = 9) and IkA16G1 (n = 4). The subtype of C. parvum was IIdA15G1, whereas the two Cryptosporidium horse genotype isolates were of subtype VIaA15G4. The predominance of C. hominis in donkeys suggests that these animals are infected through human contact. |
format | Online Article Text |
id | pubmed-7315819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-73158192020-06-29 Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China Wang, Wen Zhang, Zhenjie Zhang, Ying Zhao, Aiyun Jing, Bo Zhang, Longxian Liu, Pengtao Qi, Meng Zhao, Wei Parasite Short Note The prevalence and zoonotic potential of Cryptosporidium in donkeys is poorly understood. Here, 680 fecal specimens were collected from 178 free-ranging and 502 farmed donkeys in Xinjiang, China. Cryptosporidium was identified using PCR amplification of the small subunit of ribosomal DNA. Cryptosporidium-positive isolates were subtyped using PCR analysis of the 60 kDa glycoprotein gene (gp60). The overall prevalence of Cryptosporidium was 2.4% (16/680), with 3.2% (16/502) in farmed donkeys and 0% (0/178) in free-ranging donkeys. Cryptosporidium hominis (n = 13), C. parvum (n = 1) and Cryptosporidium horse genotype (n = 2) were identified. The C. hominis isolates belonged to two subtypes, IkA16 (n = 9) and IkA16G1 (n = 4). The subtype of C. parvum was IIdA15G1, whereas the two Cryptosporidium horse genotype isolates were of subtype VIaA15G4. The predominance of C. hominis in donkeys suggests that these animals are infected through human contact. EDP Sciences 2020-06-25 /pmc/articles/PMC7315819/ /pubmed/32584252 http://dx.doi.org/10.1051/parasite/2020042 Text en © W. Wang et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Note Wang, Wen Zhang, Zhenjie Zhang, Ying Zhao, Aiyun Jing, Bo Zhang, Longxian Liu, Pengtao Qi, Meng Zhao, Wei Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China |
title | Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China |
title_full | Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China |
title_fullStr | Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China |
title_full_unstemmed | Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China |
title_short | Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China |
title_sort | prevalence and genotypic identification of cryptosporidium in free-ranging and farm-raised donkeys (equus asinus asinus) in xinjiang, china |
topic | Short Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315819/ https://www.ncbi.nlm.nih.gov/pubmed/32584252 http://dx.doi.org/10.1051/parasite/2020042 |
work_keys_str_mv | AT wangwen prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT zhangzhenjie prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT zhangying prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT zhaoaiyun prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT jingbo prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT zhanglongxian prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT liupengtao prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT qimeng prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina AT zhaowei prevalenceandgenotypicidentificationofcryptosporidiuminfreerangingandfarmraiseddonkeysequusasinusasinusinxinjiangchina |