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Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China

The prevalence and zoonotic potential of Cryptosporidium in donkeys is poorly understood. Here, 680 fecal specimens were collected from 178 free-ranging and 502 farmed donkeys in Xinjiang, China. Cryptosporidium was identified using PCR amplification of the small subunit of ribosomal DNA. Cryptospor...

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Autores principales: Wang, Wen, Zhang, Zhenjie, Zhang, Ying, Zhao, Aiyun, Jing, Bo, Zhang, Longxian, Liu, Pengtao, Qi, Meng, Zhao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315819/
https://www.ncbi.nlm.nih.gov/pubmed/32584252
http://dx.doi.org/10.1051/parasite/2020042
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author Wang, Wen
Zhang, Zhenjie
Zhang, Ying
Zhao, Aiyun
Jing, Bo
Zhang, Longxian
Liu, Pengtao
Qi, Meng
Zhao, Wei
author_facet Wang, Wen
Zhang, Zhenjie
Zhang, Ying
Zhao, Aiyun
Jing, Bo
Zhang, Longxian
Liu, Pengtao
Qi, Meng
Zhao, Wei
author_sort Wang, Wen
collection PubMed
description The prevalence and zoonotic potential of Cryptosporidium in donkeys is poorly understood. Here, 680 fecal specimens were collected from 178 free-ranging and 502 farmed donkeys in Xinjiang, China. Cryptosporidium was identified using PCR amplification of the small subunit of ribosomal DNA. Cryptosporidium-positive isolates were subtyped using PCR analysis of the 60 kDa glycoprotein gene (gp60). The overall prevalence of Cryptosporidium was 2.4% (16/680), with 3.2% (16/502) in farmed donkeys and 0% (0/178) in free-ranging donkeys. Cryptosporidium hominis (n = 13), C. parvum (n = 1) and Cryptosporidium horse genotype (n = 2) were identified. The C. hominis isolates belonged to two subtypes, IkA16 (n = 9) and IkA16G1 (n = 4). The subtype of C. parvum was IIdA15G1, whereas the two Cryptosporidium horse genotype isolates were of subtype VIaA15G4. The predominance of C. hominis in donkeys suggests that these animals are infected through human contact.
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spelling pubmed-73158192020-06-29 Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China Wang, Wen Zhang, Zhenjie Zhang, Ying Zhao, Aiyun Jing, Bo Zhang, Longxian Liu, Pengtao Qi, Meng Zhao, Wei Parasite Short Note The prevalence and zoonotic potential of Cryptosporidium in donkeys is poorly understood. Here, 680 fecal specimens were collected from 178 free-ranging and 502 farmed donkeys in Xinjiang, China. Cryptosporidium was identified using PCR amplification of the small subunit of ribosomal DNA. Cryptosporidium-positive isolates were subtyped using PCR analysis of the 60 kDa glycoprotein gene (gp60). The overall prevalence of Cryptosporidium was 2.4% (16/680), with 3.2% (16/502) in farmed donkeys and 0% (0/178) in free-ranging donkeys. Cryptosporidium hominis (n = 13), C. parvum (n = 1) and Cryptosporidium horse genotype (n = 2) were identified. The C. hominis isolates belonged to two subtypes, IkA16 (n = 9) and IkA16G1 (n = 4). The subtype of C. parvum was IIdA15G1, whereas the two Cryptosporidium horse genotype isolates were of subtype VIaA15G4. The predominance of C. hominis in donkeys suggests that these animals are infected through human contact. EDP Sciences 2020-06-25 /pmc/articles/PMC7315819/ /pubmed/32584252 http://dx.doi.org/10.1051/parasite/2020042 Text en © W. Wang et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Note
Wang, Wen
Zhang, Zhenjie
Zhang, Ying
Zhao, Aiyun
Jing, Bo
Zhang, Longxian
Liu, Pengtao
Qi, Meng
Zhao, Wei
Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
title Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
title_full Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
title_fullStr Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
title_full_unstemmed Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
title_short Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China
title_sort prevalence and genotypic identification of cryptosporidium in free-ranging and farm-raised donkeys (equus asinus asinus) in xinjiang, china
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315819/
https://www.ncbi.nlm.nih.gov/pubmed/32584252
http://dx.doi.org/10.1051/parasite/2020042
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