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Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro
OBJECTIVE: Endometrial fibrosis, the primary pathological feature of intrauterine adhesion, may lead to disruption of endometrial tissue structure, menstrual abnormalities, infertility, and recurrent pregnancy loss. At present, no ideal therapeutic strategy exists for this fibrotic disease. Eupatili...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Reproductive Medicine
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315855/ https://www.ncbi.nlm.nih.gov/pubmed/32460455 http://dx.doi.org/10.5653/cerm.2019.03475 |
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author | Lee, Chang-Jin Hong, Seon-Hwa Yoon, Min-Ji Lee, Kyung-Ah Choi, Dong Hee Kwon, Hwang Ko, Jung-Jae Koo, Hwa Seon Kang, Youn-Jung |
author_facet | Lee, Chang-Jin Hong, Seon-Hwa Yoon, Min-Ji Lee, Kyung-Ah Choi, Dong Hee Kwon, Hwang Ko, Jung-Jae Koo, Hwa Seon Kang, Youn-Jung |
author_sort | Lee, Chang-Jin |
collection | PubMed |
description | OBJECTIVE: Endometrial fibrosis, the primary pathological feature of intrauterine adhesion, may lead to disruption of endometrial tissue structure, menstrual abnormalities, infertility, and recurrent pregnancy loss. At present, no ideal therapeutic strategy exists for this fibrotic disease. Eupatilin, a major pharmacologically active flavone from Artemisia, has been previously reported to act as a potent inducer of dedifferentiation of fibrotic tissue in the liver and lung. However, the effects of eupatilin on endometrial fibrosis have not yet been investigated. In this study, we present the first report on the impact of eupatilin treatment on transforming growth factor beta (TGF-β)-induced endometrial fibrosis. METHODS: The efficacy of eupatilin on TGF-β–induced endometrial fibrosis was assessed by examining changes in morphology and the expression levels of fibrosis markers using immunofluorescence staining and quantitative real-time reverse-transcription polymerase chain reaction. RESULTS: Eupatilin treatment significantly reduced the fibrotic activity of TGF-β–induced endometrial fibrosis in Ishikawa cells, which displayed more circular shapes and formed more colonies. Additionally, the effects of eupatilin on fibrotic markers including alpha-smooth muscle actin, hypoxia-inducible factor 1 alpha, collagen type I alpha 1 chain, and matrix metalloproteinase-2, were evaluated in TGF-β–induced endometrial fibrosis. The expression of these markers was highly upregulated by TGF-β pretreatment and recovered to the levels of control cells in response to eupatilin treatment. CONCLUSION: Our findings suggest that suppression of TGF-β–induced signaling by eupatilin might be an effective therapeutic strategy for the treatment of endometrial fibrosis. |
format | Online Article Text |
id | pubmed-7315855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Reproductive Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-73158552020-06-29 Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro Lee, Chang-Jin Hong, Seon-Hwa Yoon, Min-Ji Lee, Kyung-Ah Choi, Dong Hee Kwon, Hwang Ko, Jung-Jae Koo, Hwa Seon Kang, Youn-Jung Clin Exp Reprod Med Original Article OBJECTIVE: Endometrial fibrosis, the primary pathological feature of intrauterine adhesion, may lead to disruption of endometrial tissue structure, menstrual abnormalities, infertility, and recurrent pregnancy loss. At present, no ideal therapeutic strategy exists for this fibrotic disease. Eupatilin, a major pharmacologically active flavone from Artemisia, has been previously reported to act as a potent inducer of dedifferentiation of fibrotic tissue in the liver and lung. However, the effects of eupatilin on endometrial fibrosis have not yet been investigated. In this study, we present the first report on the impact of eupatilin treatment on transforming growth factor beta (TGF-β)-induced endometrial fibrosis. METHODS: The efficacy of eupatilin on TGF-β–induced endometrial fibrosis was assessed by examining changes in morphology and the expression levels of fibrosis markers using immunofluorescence staining and quantitative real-time reverse-transcription polymerase chain reaction. RESULTS: Eupatilin treatment significantly reduced the fibrotic activity of TGF-β–induced endometrial fibrosis in Ishikawa cells, which displayed more circular shapes and formed more colonies. Additionally, the effects of eupatilin on fibrotic markers including alpha-smooth muscle actin, hypoxia-inducible factor 1 alpha, collagen type I alpha 1 chain, and matrix metalloproteinase-2, were evaluated in TGF-β–induced endometrial fibrosis. The expression of these markers was highly upregulated by TGF-β pretreatment and recovered to the levels of control cells in response to eupatilin treatment. CONCLUSION: Our findings suggest that suppression of TGF-β–induced signaling by eupatilin might be an effective therapeutic strategy for the treatment of endometrial fibrosis. Korean Society for Reproductive Medicine 2020-06 2020-05-28 /pmc/articles/PMC7315855/ /pubmed/32460455 http://dx.doi.org/10.5653/cerm.2019.03475 Text en Copyright © 2020. THE KOREAN SOCIETY FOR REPRODUCTIVE MEDICINE This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Chang-Jin Hong, Seon-Hwa Yoon, Min-Ji Lee, Kyung-Ah Choi, Dong Hee Kwon, Hwang Ko, Jung-Jae Koo, Hwa Seon Kang, Youn-Jung Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro |
title | Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro |
title_full | Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro |
title_fullStr | Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro |
title_full_unstemmed | Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro |
title_short | Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro |
title_sort | eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315855/ https://www.ncbi.nlm.nih.gov/pubmed/32460455 http://dx.doi.org/10.5653/cerm.2019.03475 |
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