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Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China
Cryptosporidium is a ubiquitous protozoan in human and animals. To investigate the genetic diversity of Cryptosporidium spp. in alpaca (Vicugna pacos) in China, 484 fecal samples from alpacas were collected at nine sites, and Cryptosporidium spp. were screened with PCR amplification of the small sub...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316868/ https://www.ncbi.nlm.nih.gov/pubmed/32612927 http://dx.doi.org/10.1016/j.ijppaw.2020.06.007 |
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author | Zhang, Qiyuan Li, Junqiang Li, Zhiguo Xu, Chunyan Hou, Minyu Qi, Meng |
author_facet | Zhang, Qiyuan Li, Junqiang Li, Zhiguo Xu, Chunyan Hou, Minyu Qi, Meng |
author_sort | Zhang, Qiyuan |
collection | PubMed |
description | Cryptosporidium is a ubiquitous protozoan in human and animals. To investigate the genetic diversity of Cryptosporidium spp. in alpaca (Vicugna pacos) in China, 484 fecal samples from alpacas were collected at nine sites, and Cryptosporidium spp. were screened with PCR amplification of the small subunit ribosome RNA (SSU rRNA) locus. Cryptosporidium spp. infected 2.9% (14/484) of the alpacas. Of the nine collection sites, two were positive for Cryptosporidium, Wensu (3.0%, 3/100) and Qinghe (31.4%, 11/35). Three Cryptosporidium species were identified: C. parvum (n = 2), C. ubiquitum (n = 1), and C. occultus (n = 11). Cryptosporidium parvum and C. ubiquitum were further subtyped with the 60-kDa glycoprotein locus (gp60). The two C. parvum isolates were subtype IIdA15G1, but the one C. ubiquitum isolate was not subtyped successfully. A phylogenetic analysis indicated that the Cryptosporidium isolates clustered with previously identified species. To our knowledge, this is the first report of Cryptosporidium infections in alpacas in China and provides baseline data for the study of Cryptosporidium in alpacas in China. |
format | Online Article Text |
id | pubmed-7316868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-73168682020-06-30 Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China Zhang, Qiyuan Li, Junqiang Li, Zhiguo Xu, Chunyan Hou, Minyu Qi, Meng Int J Parasitol Parasites Wildl Article Cryptosporidium is a ubiquitous protozoan in human and animals. To investigate the genetic diversity of Cryptosporidium spp. in alpaca (Vicugna pacos) in China, 484 fecal samples from alpacas were collected at nine sites, and Cryptosporidium spp. were screened with PCR amplification of the small subunit ribosome RNA (SSU rRNA) locus. Cryptosporidium spp. infected 2.9% (14/484) of the alpacas. Of the nine collection sites, two were positive for Cryptosporidium, Wensu (3.0%, 3/100) and Qinghe (31.4%, 11/35). Three Cryptosporidium species were identified: C. parvum (n = 2), C. ubiquitum (n = 1), and C. occultus (n = 11). Cryptosporidium parvum and C. ubiquitum were further subtyped with the 60-kDa glycoprotein locus (gp60). The two C. parvum isolates were subtype IIdA15G1, but the one C. ubiquitum isolate was not subtyped successfully. A phylogenetic analysis indicated that the Cryptosporidium isolates clustered with previously identified species. To our knowledge, this is the first report of Cryptosporidium infections in alpacas in China and provides baseline data for the study of Cryptosporidium in alpacas in China. Elsevier 2020-06-20 /pmc/articles/PMC7316868/ /pubmed/32612927 http://dx.doi.org/10.1016/j.ijppaw.2020.06.007 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Zhang, Qiyuan Li, Junqiang Li, Zhiguo Xu, Chunyan Hou, Minyu Qi, Meng Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China |
title | Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China |
title_full | Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China |
title_fullStr | Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China |
title_full_unstemmed | Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China |
title_short | Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China |
title_sort | molecular identification of cryptosporidium spp. in alpacas (vicugna pacos) in china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316868/ https://www.ncbi.nlm.nih.gov/pubmed/32612927 http://dx.doi.org/10.1016/j.ijppaw.2020.06.007 |
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