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Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt

BACKGROUND: Neonatal sepsis is a nuisance to clinicians and medical microbiologists, particularly those cases caused by Klebsiella pneumoniae. Thus, we aimed at investigating the profile and mechanisms of antibiotic resistance and the clonal relationships between K. pneumoniae isolated from neonates...

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Autores principales: Hassuna, Noha A., AbdelAziz, Reem A., Zakaria, Amira, Abdelhakeem, Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317144/
https://www.ncbi.nlm.nih.gov/pubmed/32636828
http://dx.doi.org/10.3389/fmicb.2020.01375
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author Hassuna, Noha A.
AbdelAziz, Reem A.
Zakaria, Amira
Abdelhakeem, Mohammed
author_facet Hassuna, Noha A.
AbdelAziz, Reem A.
Zakaria, Amira
Abdelhakeem, Mohammed
author_sort Hassuna, Noha A.
collection PubMed
description BACKGROUND: Neonatal sepsis is a nuisance to clinicians and medical microbiologists, particularly those cases caused by Klebsiella pneumoniae. Thus, we aimed at investigating the profile and mechanisms of antibiotic resistance and the clonal relationships between K. pneumoniae isolated from neonates at the largest tertiary care hospital’s neonatal intensive care units (NICUs) in Minia, Egypt. METHODS: This study comprised 156 neonates diagnosed with culture-proven sepsis from February 2019 to September 2019, at a major NICU of Minia City. All K. pneumoniae isolates were collected and characterized by antimicrobial profile, resistance genotype, and pulsed-field gel electrophoresis typing. RESULTS: Twenty-four K. pneumoniae isolates (15.3%) were collected out of the 156 sepsis diagnosed neonates. These samples showed extensive drug resistance (XDR) to most of the tested antimicrobials, except fluoroquinolones. All the K. pneumoniae isolates possessed bla(VIM) and bla(NDM) carbapenemase genes, while bla(KPC) gene was detected in 95.8%. Considering extended-spectrum β-lactamases genes, bla(CTX–M) was found in all the isolates and bla(OXA–1) gene in 75% of them. The plasmid-mediated quinolone resistance gene qnrS, was predominantly found among our isolates in comparison to qnrB or qnrA. A moderate degree of clonal relatedness was observed between the isolates. CONCLUSION: To the best of our knowledge, this the first report of an alarming occurrence of XDR among K. penumoniae isolates recovered from neonatal sepsis in Egypt. Our data necessitate proper antimicrobial stewardship as the choices will be very limited.
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spelling pubmed-73171442020-07-06 Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt Hassuna, Noha A. AbdelAziz, Reem A. Zakaria, Amira Abdelhakeem, Mohammed Front Microbiol Microbiology BACKGROUND: Neonatal sepsis is a nuisance to clinicians and medical microbiologists, particularly those cases caused by Klebsiella pneumoniae. Thus, we aimed at investigating the profile and mechanisms of antibiotic resistance and the clonal relationships between K. pneumoniae isolated from neonates at the largest tertiary care hospital’s neonatal intensive care units (NICUs) in Minia, Egypt. METHODS: This study comprised 156 neonates diagnosed with culture-proven sepsis from February 2019 to September 2019, at a major NICU of Minia City. All K. pneumoniae isolates were collected and characterized by antimicrobial profile, resistance genotype, and pulsed-field gel electrophoresis typing. RESULTS: Twenty-four K. pneumoniae isolates (15.3%) were collected out of the 156 sepsis diagnosed neonates. These samples showed extensive drug resistance (XDR) to most of the tested antimicrobials, except fluoroquinolones. All the K. pneumoniae isolates possessed bla(VIM) and bla(NDM) carbapenemase genes, while bla(KPC) gene was detected in 95.8%. Considering extended-spectrum β-lactamases genes, bla(CTX–M) was found in all the isolates and bla(OXA–1) gene in 75% of them. The plasmid-mediated quinolone resistance gene qnrS, was predominantly found among our isolates in comparison to qnrB or qnrA. A moderate degree of clonal relatedness was observed between the isolates. CONCLUSION: To the best of our knowledge, this the first report of an alarming occurrence of XDR among K. penumoniae isolates recovered from neonatal sepsis in Egypt. Our data necessitate proper antimicrobial stewardship as the choices will be very limited. Frontiers Media S.A. 2020-06-19 /pmc/articles/PMC7317144/ /pubmed/32636828 http://dx.doi.org/10.3389/fmicb.2020.01375 Text en Copyright © 2020 Hassuna, AbdelAziz, Zakaria and Abdelhakeem. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hassuna, Noha A.
AbdelAziz, Reem A.
Zakaria, Amira
Abdelhakeem, Mohammed
Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt
title Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt
title_full Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt
title_fullStr Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt
title_full_unstemmed Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt
title_short Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt
title_sort extensively-drug resistant klebsiella pneumoniae recovered from neonatal sepsis cases from a major nicu in egypt
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317144/
https://www.ncbi.nlm.nih.gov/pubmed/32636828
http://dx.doi.org/10.3389/fmicb.2020.01375
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