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Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth.
Kigelia africana is used to manage inflammation among indigenous communities. We hypothesized that K. africana extracts contain phytoconstituents with good antioxidant and anti-inflammatory activities. The methanolic extract of K. africana fruits and Spathodea campanulata leaves (SPK04), K. africana...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317318/ https://www.ncbi.nlm.nih.gov/pubmed/32655661 http://dx.doi.org/10.1155/2020/4352084 |
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author | Nabatanzi, Alice Nkadimeng, Sanah M. Lall, Namrita Kabasa, John D. McGaw, Lyndy J. |
author_facet | Nabatanzi, Alice Nkadimeng, Sanah M. Lall, Namrita Kabasa, John D. McGaw, Lyndy J. |
author_sort | Nabatanzi, Alice |
collection | PubMed |
description | Kigelia africana is used to manage inflammation among indigenous communities. We hypothesized that K. africana extracts contain phytoconstituents with good antioxidant and anti-inflammatory activities. The methanolic extract of K. africana fruits and Spathodea campanulata leaves (SPK04), K. africana aqueous fruit extract (KFM02), and K. africana acetone fruit extract (KFM05) were subjected to antioxidant and anti-inflammatory assays. Antioxidant activity was evaluated using the ABTS radical scavenging assay, and the MTT cell viability assay was used for cytotoxicity. The extracts were preincubated with enzymes and assayed for 15-LOX and COX-2 enzyme activity using an ELISA method. Nitric oxide (NO) inhibitory effect of the extracts was evaluated and measurement of proinflammatory cytokines (IL-1β, TNF-α, and IL-6) and the anti-inflammatory cytokine (IL-10) was done using ELISA kits. SPK04 had the highest antioxidant activity with a mean inhibition of 99.37 ± 0.56% and an IC(50) of 4.28 µg/mL. SPK04 and KFM05 did not inhibit 15-LOX as their IC(50) values were >1000 μg/mL. All extracts were safe on Vero cells at the highest concentration (200 µg/mL) tested. KFM02 was the best inhibitor of NO production and had the highest cell viability at both the lowest (50 µg/mL) and highest concentrations (200 µg/mL). SPK04 was the best COX-2 inhibitor while KFM05 expressed the strongest suppression effect for IL-β and IL-6. KFM02 did not inhibit IL-6 at the highest concentration (200 µg/mL). The order of suppression of TNF-α by the extracts differed across concentrations, KFM05 > SPK04 > KFM02 at 200 µg/mL, KFM02 > SPK04 > KFM05 at 100 µg/mL, and SPK04 > KFM02 > KFM05 at 50 µg/mL. All the tested extracts had no inhibitory effect against IL-10. SPK04, KFM05, and KFM02 had good antioxidant and anti-inflammatory activity and this supports their use as potential anti-inflammatory therapies. This study presents for the first time the antioxidant and anti-inflammatory activity of K. africana and S. campanulata polyherbal extract. It is also among the very few studies that have reported the inhibitory effect of cytokines IL-1β, TNF-α, IL-6, and IL-10 by K. africana. |
format | Online Article Text |
id | pubmed-7317318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-73173182020-07-11 Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth. Nabatanzi, Alice Nkadimeng, Sanah M. Lall, Namrita Kabasa, John D. McGaw, Lyndy J. Evid Based Complement Alternat Med Research Article Kigelia africana is used to manage inflammation among indigenous communities. We hypothesized that K. africana extracts contain phytoconstituents with good antioxidant and anti-inflammatory activities. The methanolic extract of K. africana fruits and Spathodea campanulata leaves (SPK04), K. africana aqueous fruit extract (KFM02), and K. africana acetone fruit extract (KFM05) were subjected to antioxidant and anti-inflammatory assays. Antioxidant activity was evaluated using the ABTS radical scavenging assay, and the MTT cell viability assay was used for cytotoxicity. The extracts were preincubated with enzymes and assayed for 15-LOX and COX-2 enzyme activity using an ELISA method. Nitric oxide (NO) inhibitory effect of the extracts was evaluated and measurement of proinflammatory cytokines (IL-1β, TNF-α, and IL-6) and the anti-inflammatory cytokine (IL-10) was done using ELISA kits. SPK04 had the highest antioxidant activity with a mean inhibition of 99.37 ± 0.56% and an IC(50) of 4.28 µg/mL. SPK04 and KFM05 did not inhibit 15-LOX as their IC(50) values were >1000 μg/mL. All extracts were safe on Vero cells at the highest concentration (200 µg/mL) tested. KFM02 was the best inhibitor of NO production and had the highest cell viability at both the lowest (50 µg/mL) and highest concentrations (200 µg/mL). SPK04 was the best COX-2 inhibitor while KFM05 expressed the strongest suppression effect for IL-β and IL-6. KFM02 did not inhibit IL-6 at the highest concentration (200 µg/mL). The order of suppression of TNF-α by the extracts differed across concentrations, KFM05 > SPK04 > KFM02 at 200 µg/mL, KFM02 > SPK04 > KFM05 at 100 µg/mL, and SPK04 > KFM02 > KFM05 at 50 µg/mL. All the tested extracts had no inhibitory effect against IL-10. SPK04, KFM05, and KFM02 had good antioxidant and anti-inflammatory activity and this supports their use as potential anti-inflammatory therapies. This study presents for the first time the antioxidant and anti-inflammatory activity of K. africana and S. campanulata polyherbal extract. It is also among the very few studies that have reported the inhibitory effect of cytokines IL-1β, TNF-α, IL-6, and IL-10 by K. africana. Hindawi 2020-06-17 /pmc/articles/PMC7317318/ /pubmed/32655661 http://dx.doi.org/10.1155/2020/4352084 Text en Copyright © 2020 Alice Nabatanzi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nabatanzi, Alice Nkadimeng, Sanah M. Lall, Namrita Kabasa, John D. McGaw, Lyndy J. Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth. |
title | Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth. |
title_full | Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth. |
title_fullStr | Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth. |
title_full_unstemmed | Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth. |
title_short | Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth. |
title_sort | antioxidant and anti-inflammatory activities of kigelia africana (lam.) benth. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317318/ https://www.ncbi.nlm.nih.gov/pubmed/32655661 http://dx.doi.org/10.1155/2020/4352084 |
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