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An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria

Herein we report the development of a turn‐on lanthanide luminescent probe for time‐gated detection of nitroreductases (NTRs) in live bacteria. The probe is activated through NTR‐induced formation of the sensitizing carbostyril antenna and resulting energy transfer to the lanthanide center. This nov...

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Autores principales: Brennecke, Benjamin, Wang, Qinghua, Zhang, Qingyang, Hu, Hai‐Yu, Nazaré, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317344/
https://www.ncbi.nlm.nih.gov/pubmed/32212410
http://dx.doi.org/10.1002/anie.202002391
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author Brennecke, Benjamin
Wang, Qinghua
Zhang, Qingyang
Hu, Hai‐Yu
Nazaré, Marc
author_facet Brennecke, Benjamin
Wang, Qinghua
Zhang, Qingyang
Hu, Hai‐Yu
Nazaré, Marc
author_sort Brennecke, Benjamin
collection PubMed
description Herein we report the development of a turn‐on lanthanide luminescent probe for time‐gated detection of nitroreductases (NTRs) in live bacteria. The probe is activated through NTR‐induced formation of the sensitizing carbostyril antenna and resulting energy transfer to the lanthanide center. This novel NTR‐responsive trigger is virtually non‐fluorescent in its inactivated form and features a large signal increase upon activation. We show that the probe is capable of selectively sensing NTR in lysates as well as in live bacteria of the ESKAPE family which are clinically highly relevant multiresistant pathogens responsible for the majority of hospital infections. The results suggest that our probe could be used to develop diagnostic tools for bacterial infections.
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spelling pubmed-73173442020-06-30 An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria Brennecke, Benjamin Wang, Qinghua Zhang, Qingyang Hu, Hai‐Yu Nazaré, Marc Angew Chem Int Ed Engl Communications Herein we report the development of a turn‐on lanthanide luminescent probe for time‐gated detection of nitroreductases (NTRs) in live bacteria. The probe is activated through NTR‐induced formation of the sensitizing carbostyril antenna and resulting energy transfer to the lanthanide center. This novel NTR‐responsive trigger is virtually non‐fluorescent in its inactivated form and features a large signal increase upon activation. We show that the probe is capable of selectively sensing NTR in lysates as well as in live bacteria of the ESKAPE family which are clinically highly relevant multiresistant pathogens responsible for the majority of hospital infections. The results suggest that our probe could be used to develop diagnostic tools for bacterial infections. John Wiley and Sons Inc. 2020-04-16 2020-05-25 /pmc/articles/PMC7317344/ /pubmed/32212410 http://dx.doi.org/10.1002/anie.202002391 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Brennecke, Benjamin
Wang, Qinghua
Zhang, Qingyang
Hu, Hai‐Yu
Nazaré, Marc
An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria
title An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria
title_full An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria
title_fullStr An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria
title_full_unstemmed An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria
title_short An Activatable Lanthanide Luminescent Probe for Time‐Gated Detection of Nitroreductase in Live Bacteria
title_sort activatable lanthanide luminescent probe for time‐gated detection of nitroreductase in live bacteria
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317344/
https://www.ncbi.nlm.nih.gov/pubmed/32212410
http://dx.doi.org/10.1002/anie.202002391
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