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Tuning Enzyme Activity for Nonaqueous Solvents: Engineering an Enantioselective “Michaelase” for Catalysis in High Concentrations of Ethanol
Enzymes have evolved to function under aqueous conditions and may not exhibit features essential for biocatalytic application, such as the ability to function in high concentrations of an organic solvent. Consequently, protein engineering is often required to tune an enzyme for catalysis in non‐aque...
Autores principales: | Guo, Chao, Biewenga, Lieuwe, Lubberink, Max, van Merkerk, Ronald, Poelarends, Gerrit J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317446/ https://www.ncbi.nlm.nih.gov/pubmed/31886617 http://dx.doi.org/10.1002/cbic.201900721 |
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