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Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA

Campylobacter jejuni is a bacterial pathogen that is generally acquired as a zoonotic infection from poultry and animals. Adhesion of C. jejuni to human colorectal epithelial cells is weakened after loss of its cj0588 gene. The Cj0588 protein belongs to the type I group of TlyA (TlyA(I)) enzymes, wh...

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Autores principales: Sałamaszyńska‐Guz, Agnieszka, Serafińska, Izabela, Bącal, Paweł, Douthwaite, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317525/
https://www.ncbi.nlm.nih.gov/pubmed/32134554
http://dx.doi.org/10.1111/cmi.13199
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author Sałamaszyńska‐Guz, Agnieszka
Serafińska, Izabela
Bącal, Paweł
Douthwaite, Stephen
author_facet Sałamaszyńska‐Guz, Agnieszka
Serafińska, Izabela
Bącal, Paweł
Douthwaite, Stephen
author_sort Sałamaszyńska‐Guz, Agnieszka
collection PubMed
description Campylobacter jejuni is a bacterial pathogen that is generally acquired as a zoonotic infection from poultry and animals. Adhesion of C. jejuni to human colorectal epithelial cells is weakened after loss of its cj0588 gene. The Cj0588 protein belongs to the type I group of TlyA (TlyA(I)) enzymes, which 2′‐O‐methylate nucleotide C1920 in 23S rRNA. Slightly longer TlyA(II) versions of the methyltransferase are found in actinobacterial species including Mycobacterium tuberculosis, and methylate not only C1920 but also nucleotide C1409 in 16S rRNA. Loss of TlyA function attenuates virulence of both M. tuberculosis and C. jejuni. We show here that the traits impaired in C. jejuni null strains can be rescued by complementation not only with the original cj0588 (tlyA (I) ) but also with a mycobacterial tlyA (II) gene. There are, however, significant differences in the recombinant phenotypes. While cj0588 restores motility, biofilm formation, adhesion to and invasion of human epithelial cells and stimulation of IL‐8 production in a C. jejuni null strain, several of these properties are further enhanced by the mycobacterial tlyA (II) gene, in some cases to twice the original wild‐type level. These findings strongly suggest that subtle changes in rRNA modification patterns can affect protein synthesis in a manner that has serious consequences for bacterial pathogenicity.
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spelling pubmed-73175252020-06-29 Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA Sałamaszyńska‐Guz, Agnieszka Serafińska, Izabela Bącal, Paweł Douthwaite, Stephen Cell Microbiol Research Articles Campylobacter jejuni is a bacterial pathogen that is generally acquired as a zoonotic infection from poultry and animals. Adhesion of C. jejuni to human colorectal epithelial cells is weakened after loss of its cj0588 gene. The Cj0588 protein belongs to the type I group of TlyA (TlyA(I)) enzymes, which 2′‐O‐methylate nucleotide C1920 in 23S rRNA. Slightly longer TlyA(II) versions of the methyltransferase are found in actinobacterial species including Mycobacterium tuberculosis, and methylate not only C1920 but also nucleotide C1409 in 16S rRNA. Loss of TlyA function attenuates virulence of both M. tuberculosis and C. jejuni. We show here that the traits impaired in C. jejuni null strains can be rescued by complementation not only with the original cj0588 (tlyA (I) ) but also with a mycobacterial tlyA (II) gene. There are, however, significant differences in the recombinant phenotypes. While cj0588 restores motility, biofilm formation, adhesion to and invasion of human epithelial cells and stimulation of IL‐8 production in a C. jejuni null strain, several of these properties are further enhanced by the mycobacterial tlyA (II) gene, in some cases to twice the original wild‐type level. These findings strongly suggest that subtle changes in rRNA modification patterns can affect protein synthesis in a manner that has serious consequences for bacterial pathogenicity. John Wiley & Sons, Inc. 2020-03-24 2020-07 /pmc/articles/PMC7317525/ /pubmed/32134554 http://dx.doi.org/10.1111/cmi.13199 Text en © 2020 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Sałamaszyńska‐Guz, Agnieszka
Serafińska, Izabela
Bącal, Paweł
Douthwaite, Stephen
Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA
title Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA
title_full Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA
title_fullStr Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA
title_full_unstemmed Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA
title_short Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA
title_sort virulence properties of campylobacter jejuni are enhanced by displaying a mycobacterial tlya methylation pattern in its rrna
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317525/
https://www.ncbi.nlm.nih.gov/pubmed/32134554
http://dx.doi.org/10.1111/cmi.13199
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