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Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy

OBJECTIVES: To determine the feasibility and tissue yield of a perinatal incisionless ultrasound‐guided biopsy procedure, the INcisionless Targeted Core Tissue (INTACT) technique, in the context of minimally invasive autopsy. METHODS: Cases of perinatal death in which the parents consented for minim...

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Autores principales: Shelmerdine, S. C., Hutchinson, J. C., Ward, L., Sekar, T., Ashworth, M. T., Levine, S., Sebire, N. J., Arthurs, O. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317589/
https://www.ncbi.nlm.nih.gov/pubmed/31271478
http://dx.doi.org/10.1002/uog.20387
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author Shelmerdine, S. C.
Hutchinson, J. C.
Ward, L.
Sekar, T.
Ashworth, M. T.
Levine, S.
Sebire, N. J.
Arthurs, O. J.
author_facet Shelmerdine, S. C.
Hutchinson, J. C.
Ward, L.
Sekar, T.
Ashworth, M. T.
Levine, S.
Sebire, N. J.
Arthurs, O. J.
author_sort Shelmerdine, S. C.
collection PubMed
description OBJECTIVES: To determine the feasibility and tissue yield of a perinatal incisionless ultrasound‐guided biopsy procedure, the INcisionless Targeted Core Tissue (INTACT) technique, in the context of minimally invasive autopsy. METHODS: Cases of perinatal death in which the parents consented for minimally invasive autopsy underwent postmortem magnetic resonance imaging and an INTACT biopsy procedure, defined as needle biopsy of organs via the umbilical cord, performed under ultrasound guidance. In each case, three cores of tissue were obtained from seven target organs (both lungs, both kidneys, heart, spleen and liver). Biopsy success was predefined as an adequate volume of the intended target organ for pathological analysis, as judged by a pathologist blinded to the case and biopsy procedure. RESULTS: Thirty fetuses underwent organ sampling. Mean gestational age was 30 weeks (range, 18–40 weeks) and mean delivery‐to‐biopsy interval was 12 days (range, 6–22 days). The overall biopsy success rate was 153/201 (76.1%) samples, with the success rates in individual organs being highest for the heart and lungs (93% and 91%, respectively) and lowest for the spleen (11%). Excluding splenic samples, the biopsy success rate was 150/173 (86.7%). Histological abnormalities were found in 4/201 (2%) samples, all of which occurred in the lungs and kidneys of a fetus with pulmonary hypoplasia and multicystic kidney disease. CONCLUSIONS: Incisionless ultrasound‐guided organ biopsy using the INTACT procedure is feasible, with an overall biopsy success rate of over 75%. This novel technique offers the ideal combination of an imaging‐led autopsy with organ sampling for parents who decline the conventional invasive approach. © 2019 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of the International Society of Ultrasound in Obstetrics and Gynecology.
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spelling pubmed-73175892020-06-29 Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy Shelmerdine, S. C. Hutchinson, J. C. Ward, L. Sekar, T. Ashworth, M. T. Levine, S. Sebire, N. J. Arthurs, O. J. Ultrasound Obstet Gynecol Original Papers OBJECTIVES: To determine the feasibility and tissue yield of a perinatal incisionless ultrasound‐guided biopsy procedure, the INcisionless Targeted Core Tissue (INTACT) technique, in the context of minimally invasive autopsy. METHODS: Cases of perinatal death in which the parents consented for minimally invasive autopsy underwent postmortem magnetic resonance imaging and an INTACT biopsy procedure, defined as needle biopsy of organs via the umbilical cord, performed under ultrasound guidance. In each case, three cores of tissue were obtained from seven target organs (both lungs, both kidneys, heart, spleen and liver). Biopsy success was predefined as an adequate volume of the intended target organ for pathological analysis, as judged by a pathologist blinded to the case and biopsy procedure. RESULTS: Thirty fetuses underwent organ sampling. Mean gestational age was 30 weeks (range, 18–40 weeks) and mean delivery‐to‐biopsy interval was 12 days (range, 6–22 days). The overall biopsy success rate was 153/201 (76.1%) samples, with the success rates in individual organs being highest for the heart and lungs (93% and 91%, respectively) and lowest for the spleen (11%). Excluding splenic samples, the biopsy success rate was 150/173 (86.7%). Histological abnormalities were found in 4/201 (2%) samples, all of which occurred in the lungs and kidneys of a fetus with pulmonary hypoplasia and multicystic kidney disease. CONCLUSIONS: Incisionless ultrasound‐guided organ biopsy using the INTACT procedure is feasible, with an overall biopsy success rate of over 75%. This novel technique offers the ideal combination of an imaging‐led autopsy with organ sampling for parents who decline the conventional invasive approach. © 2019 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of the International Society of Ultrasound in Obstetrics and Gynecology. John Wiley & Sons, Ltd. 2020-05-01 2020-05 /pmc/articles/PMC7317589/ /pubmed/31271478 http://dx.doi.org/10.1002/uog.20387 Text en © 2019 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of the International Society of Ultrasound in Obstetrics and Gynecology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Papers
Shelmerdine, S. C.
Hutchinson, J. C.
Ward, L.
Sekar, T.
Ashworth, M. T.
Levine, S.
Sebire, N. J.
Arthurs, O. J.
Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy
title Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy
title_full Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy
title_fullStr Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy
title_full_unstemmed Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy
title_short Feasibility of INTACT (INcisionless TArgeted Core Tissue) biopsy procedure for perinatal autopsy
title_sort feasibility of intact (incisionless targeted core tissue) biopsy procedure for perinatal autopsy
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317589/
https://www.ncbi.nlm.nih.gov/pubmed/31271478
http://dx.doi.org/10.1002/uog.20387
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