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Intact and middle‐down CIEF of commercial therapeutic monoclonal antibody products under non‐denaturing conditions
A two‐step CIEF with chemical mobilization was developed for charge profiling of the therapeutic mAb rituximab under non‐denaturing separation conditions. CIEF of the intact mAb was combined with a middle‐down approach analyzing Fc/2 and F(ab´)(2) fragments after digest with a commercial cysteine pr...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317833/ https://www.ncbi.nlm.nih.gov/pubmed/32250465 http://dx.doi.org/10.1002/elps.202000013 |