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Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology
Three different types of electron‐transferring metallo‐ATPases are able to couple ATP hydrolysis to the reduction of low‐potential metal sites, thereby energizing an electron. Besides the Fe‐protein known from nitrogenase and homologous enzymes, two other kinds of ATPase with different scaffolds and...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317905/ https://www.ncbi.nlm.nih.gov/pubmed/32187824 http://dx.doi.org/10.1002/cbic.202000016 |
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author | Jeoung, Jae‐Hun Martins, Berta M. Dobbek, Holger |
author_facet | Jeoung, Jae‐Hun Martins, Berta M. Dobbek, Holger |
author_sort | Jeoung, Jae‐Hun |
collection | PubMed |
description | Three different types of electron‐transferring metallo‐ATPases are able to couple ATP hydrolysis to the reduction of low‐potential metal sites, thereby energizing an electron. Besides the Fe‐protein known from nitrogenase and homologous enzymes, two other kinds of ATPase with different scaffolds and cofactors are used to achieve a unidirectional, energetic, uphill electron transfer to either reduce inactive Co‐corrinoid‐containing proteins (RACE‐type activators) or a second iron‐sulfur cluster‐containing enzyme of a unique radical enzymes family (archerases). We have found a new cofactor in the latter enzyme family, that is, a double‐cubane cluster with two [4Fe4S] subclusters bridged by a sulfido ligand. An enzyme containing this cofactor catalyzes the ATP‐dependent reduction of small molecules, including acetylene. Thus, enzymes containing the double‐cubane cofactor are analogous in function and share some structural features with nitrogenases. |
format | Online Article Text |
id | pubmed-7317905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73179052020-06-29 Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology Jeoung, Jae‐Hun Martins, Berta M. Dobbek, Holger Chembiochem Minireviews Three different types of electron‐transferring metallo‐ATPases are able to couple ATP hydrolysis to the reduction of low‐potential metal sites, thereby energizing an electron. Besides the Fe‐protein known from nitrogenase and homologous enzymes, two other kinds of ATPase with different scaffolds and cofactors are used to achieve a unidirectional, energetic, uphill electron transfer to either reduce inactive Co‐corrinoid‐containing proteins (RACE‐type activators) or a second iron‐sulfur cluster‐containing enzyme of a unique radical enzymes family (archerases). We have found a new cofactor in the latter enzyme family, that is, a double‐cubane cluster with two [4Fe4S] subclusters bridged by a sulfido ligand. An enzyme containing this cofactor catalyzes the ATP‐dependent reduction of small molecules, including acetylene. Thus, enzymes containing the double‐cubane cofactor are analogous in function and share some structural features with nitrogenases. John Wiley and Sons Inc. 2020-03-20 2020-06-15 /pmc/articles/PMC7317905/ /pubmed/32187824 http://dx.doi.org/10.1002/cbic.202000016 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Minireviews Jeoung, Jae‐Hun Martins, Berta M. Dobbek, Holger Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology |
title | Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology |
title_full | Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology |
title_fullStr | Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology |
title_full_unstemmed | Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology |
title_short | Double‐Cubane [8Fe9S] Clusters: A Novel Nitrogenase‐Related Cofactor in Biology |
title_sort | double‐cubane [8fe9s] clusters: a novel nitrogenase‐related cofactor in biology |
topic | Minireviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317905/ https://www.ncbi.nlm.nih.gov/pubmed/32187824 http://dx.doi.org/10.1002/cbic.202000016 |
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