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Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation

In bone tissue engineering (TE), an efficient seeding and homogenous distribution of cells is needed to avoid cell loss and damage as well as to facilitate tissue development. Dynamic seeding methods seem to be superior to the static ones because they tend to result in a more homogeneous cell distri...

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Autores principales: Melke, Johanna, Zhao, Feihu, Ito, Keita, Hofmann, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317919/
https://www.ncbi.nlm.nih.gov/pubmed/31922286
http://dx.doi.org/10.1002/jor.24583
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author Melke, Johanna
Zhao, Feihu
Ito, Keita
Hofmann, Sandra
author_facet Melke, Johanna
Zhao, Feihu
Ito, Keita
Hofmann, Sandra
author_sort Melke, Johanna
collection PubMed
description In bone tissue engineering (TE), an efficient seeding and homogenous distribution of cells is needed to avoid cell loss and damage as well as to facilitate tissue development. Dynamic seeding methods seem to be superior to the static ones because they tend to result in a more homogeneous cell distribution by using kinetic forces. However, most dynamic seeding techniques are elaborate or require special equipment and its influence on the final bone tissue‐engineered construct is not clear. In this study, we applied a simple, dynamic seeding method using an orbital shaker to seed human bone marrow–derived mesenchymal stromal cells (hBMSCs) on silk fibroin scaffolds. Significantly higher cell numbers with a more homogenous cell distribution, increased osteogenic differentiation, and mineral deposition were observed using the dynamic approach both for 4 and 6 hours as compared to the static seeding method. The positive influence of dynamic seeding could be attributed to both cell density and distribution but also nutrient supply during seeding and shear stresses (0.0‐3.0 mPa) as determined by computational simulations. The influence of relevant mechanical stimuli during seeding should be investigated in the future, especially regarding the importance of mechanical cues for bone TE applications. Our results highlight the importance of adequate choice of seeding method and its impact on developing tissue‐engineered constructs. The application of this simple seeding technique is not only recommended for bone TE but can also be used for seeding similar porous scaffolds with hBMSCs in other TE fields.
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spelling pubmed-73179192020-06-29 Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation Melke, Johanna Zhao, Feihu Ito, Keita Hofmann, Sandra J Orthop Res Research Articles In bone tissue engineering (TE), an efficient seeding and homogenous distribution of cells is needed to avoid cell loss and damage as well as to facilitate tissue development. Dynamic seeding methods seem to be superior to the static ones because they tend to result in a more homogeneous cell distribution by using kinetic forces. However, most dynamic seeding techniques are elaborate or require special equipment and its influence on the final bone tissue‐engineered construct is not clear. In this study, we applied a simple, dynamic seeding method using an orbital shaker to seed human bone marrow–derived mesenchymal stromal cells (hBMSCs) on silk fibroin scaffolds. Significantly higher cell numbers with a more homogenous cell distribution, increased osteogenic differentiation, and mineral deposition were observed using the dynamic approach both for 4 and 6 hours as compared to the static seeding method. The positive influence of dynamic seeding could be attributed to both cell density and distribution but also nutrient supply during seeding and shear stresses (0.0‐3.0 mPa) as determined by computational simulations. The influence of relevant mechanical stimuli during seeding should be investigated in the future, especially regarding the importance of mechanical cues for bone TE applications. Our results highlight the importance of adequate choice of seeding method and its impact on developing tissue‐engineered constructs. The application of this simple seeding technique is not only recommended for bone TE but can also be used for seeding similar porous scaffolds with hBMSCs in other TE fields. John Wiley and Sons Inc. 2020-01-14 2020-06 /pmc/articles/PMC7317919/ /pubmed/31922286 http://dx.doi.org/10.1002/jor.24583 Text en © 2020 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Melke, Johanna
Zhao, Feihu
Ito, Keita
Hofmann, Sandra
Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation
title Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation
title_full Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation
title_fullStr Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation
title_full_unstemmed Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation
title_short Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation
title_sort orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone‐like tissue formation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317919/
https://www.ncbi.nlm.nih.gov/pubmed/31922286
http://dx.doi.org/10.1002/jor.24583
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