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Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria
BACKGROUND: Malaria in sub-Saharan Africa (sSA) is thought to be mostly caused by Plasmodium falciparum. Recently, growing reports of cases due to Plasmodium ovale, Plasmodium malariae, and Plasmodium vivax have been increasingly observed to play a role in malaria epidemiology in sSA. This in fact i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318376/ https://www.ncbi.nlm.nih.gov/pubmed/32590997 http://dx.doi.org/10.1186/s12936-020-03301-w |
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author | Oboh, Mary Aigbiremo Singh, Upasana Shyamsunder Ndiaye, Daouda Badiane, Aida Sadikh Ali, Nazia Anwar Bharti, Praveen Kumar Das, Aparup |
author_facet | Oboh, Mary Aigbiremo Singh, Upasana Shyamsunder Ndiaye, Daouda Badiane, Aida Sadikh Ali, Nazia Anwar Bharti, Praveen Kumar Das, Aparup |
author_sort | Oboh, Mary Aigbiremo |
collection | PubMed |
description | BACKGROUND: Malaria in sub-Saharan Africa (sSA) is thought to be mostly caused by Plasmodium falciparum. Recently, growing reports of cases due to Plasmodium ovale, Plasmodium malariae, and Plasmodium vivax have been increasingly observed to play a role in malaria epidemiology in sSA. This in fact is due to the usage of very sensitive diagnostic tools (e.g. PCR), which have highlighted the underestimation of non-falciparum malaria in this sub-region. Plasmodium vivax was historically thought to be absent in sSA due to the high prevalence of the Duffy negativity in individuals residing in this sub-continent. Recent studies reporting detection of vivax malaria in Duffy-negative individuals from Mali, Mauritania, Cameroon challenge this notion. METHODS: Following previous report of P. vivax in Duffy-negative individuals in Nigeria, samples were further collected and assessed RDT and/or microscopy. Thereafter, malaria positive samples were subjected to conventional PCR method and DNA sequencing to confirm both single/mixed infections as well as the Duffy status of the individuals. RESULTS: Amplification of Plasmodium gDNA was successful in 59.9% (145/242) of the evaluated isolates and as expected P. falciparum was the most predominant (91.7%) species identified. Interestingly, four P. vivax isolates were identified either as single (3) or mixed (one P. falciparum/P. vivax) infection. Sequencing results confirmed all vivax isolates as truly vivax malaria and the patient were of Duffy-negative genotype. CONCLUSION: Identification of additional vivax isolates among Duffy-negative individuals from Nigeria, substantiate the expanding body of evidence on the ability of P. vivax to infect RBCs that do not express the DARC gene. Hence, such genetic-epidemiological study should be conducted at the country level in order to evaluate the true burden of P. vivax in Nigeria. |
format | Online Article Text |
id | pubmed-7318376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73183762020-06-29 Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria Oboh, Mary Aigbiremo Singh, Upasana Shyamsunder Ndiaye, Daouda Badiane, Aida Sadikh Ali, Nazia Anwar Bharti, Praveen Kumar Das, Aparup Malar J Research BACKGROUND: Malaria in sub-Saharan Africa (sSA) is thought to be mostly caused by Plasmodium falciparum. Recently, growing reports of cases due to Plasmodium ovale, Plasmodium malariae, and Plasmodium vivax have been increasingly observed to play a role in malaria epidemiology in sSA. This in fact is due to the usage of very sensitive diagnostic tools (e.g. PCR), which have highlighted the underestimation of non-falciparum malaria in this sub-region. Plasmodium vivax was historically thought to be absent in sSA due to the high prevalence of the Duffy negativity in individuals residing in this sub-continent. Recent studies reporting detection of vivax malaria in Duffy-negative individuals from Mali, Mauritania, Cameroon challenge this notion. METHODS: Following previous report of P. vivax in Duffy-negative individuals in Nigeria, samples were further collected and assessed RDT and/or microscopy. Thereafter, malaria positive samples were subjected to conventional PCR method and DNA sequencing to confirm both single/mixed infections as well as the Duffy status of the individuals. RESULTS: Amplification of Plasmodium gDNA was successful in 59.9% (145/242) of the evaluated isolates and as expected P. falciparum was the most predominant (91.7%) species identified. Interestingly, four P. vivax isolates were identified either as single (3) or mixed (one P. falciparum/P. vivax) infection. Sequencing results confirmed all vivax isolates as truly vivax malaria and the patient were of Duffy-negative genotype. CONCLUSION: Identification of additional vivax isolates among Duffy-negative individuals from Nigeria, substantiate the expanding body of evidence on the ability of P. vivax to infect RBCs that do not express the DARC gene. Hence, such genetic-epidemiological study should be conducted at the country level in order to evaluate the true burden of P. vivax in Nigeria. BioMed Central 2020-06-26 /pmc/articles/PMC7318376/ /pubmed/32590997 http://dx.doi.org/10.1186/s12936-020-03301-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Oboh, Mary Aigbiremo Singh, Upasana Shyamsunder Ndiaye, Daouda Badiane, Aida Sadikh Ali, Nazia Anwar Bharti, Praveen Kumar Das, Aparup Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria |
title | Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria |
title_full | Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria |
title_fullStr | Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria |
title_full_unstemmed | Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria |
title_short | Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria |
title_sort | presence of additional plasmodium vivax malaria in duffy negative individuals from southwestern nigeria |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318376/ https://www.ncbi.nlm.nih.gov/pubmed/32590997 http://dx.doi.org/10.1186/s12936-020-03301-w |
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