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Resolving Binding Events on the Multifunctional Human Serum Albumin
Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318646/ https://www.ncbi.nlm.nih.gov/pubmed/32162429 http://dx.doi.org/10.1002/cmdc.202000069 |
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author | Wenskowsky, Lea Wagner, Michael Reusch, Johannes Schreuder, Herman Matter, Hans Opatz, Till Petry, Stefan Matthias |
author_facet | Wenskowsky, Lea Wagner, Michael Reusch, Johannes Schreuder, Herman Matter, Hans Opatz, Till Petry, Stefan Matthias |
author_sort | Wenskowsky, Lea |
collection | PubMed |
description | Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding‐ and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X‐ray crystallography and time‐resolved dynamics measurements (switchSENSE technology) confirm the validity of the assay, the localization of binding sites and furthermore reveal conformational changes associated with ligand binding. The described assay system allows for the detailed characterization of albumin‐binding drugs and is therefore well‐suited for prediction of drug‐drug and drug‐food interactions. Moreover, conformational changes, usually associated with binding events, can also be analyzed. |
format | Online Article Text |
id | pubmed-7318646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73186462020-06-29 Resolving Binding Events on the Multifunctional Human Serum Albumin Wenskowsky, Lea Wagner, Michael Reusch, Johannes Schreuder, Herman Matter, Hans Opatz, Till Petry, Stefan Matthias ChemMedChem Communications Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding‐ and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X‐ray crystallography and time‐resolved dynamics measurements (switchSENSE technology) confirm the validity of the assay, the localization of binding sites and furthermore reveal conformational changes associated with ligand binding. The described assay system allows for the detailed characterization of albumin‐binding drugs and is therefore well‐suited for prediction of drug‐drug and drug‐food interactions. Moreover, conformational changes, usually associated with binding events, can also be analyzed. John Wiley and Sons Inc. 2020-03-19 2020-05-06 /pmc/articles/PMC7318646/ /pubmed/32162429 http://dx.doi.org/10.1002/cmdc.202000069 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Communications Wenskowsky, Lea Wagner, Michael Reusch, Johannes Schreuder, Herman Matter, Hans Opatz, Till Petry, Stefan Matthias Resolving Binding Events on the Multifunctional Human Serum Albumin |
title | Resolving Binding Events on the Multifunctional Human Serum Albumin |
title_full | Resolving Binding Events on the Multifunctional Human Serum Albumin |
title_fullStr | Resolving Binding Events on the Multifunctional Human Serum Albumin |
title_full_unstemmed | Resolving Binding Events on the Multifunctional Human Serum Albumin |
title_short | Resolving Binding Events on the Multifunctional Human Serum Albumin |
title_sort | resolving binding events on the multifunctional human serum albumin |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318646/ https://www.ncbi.nlm.nih.gov/pubmed/32162429 http://dx.doi.org/10.1002/cmdc.202000069 |
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