Cargando…

Proton‐Detected Solid‐State NMR of the Cell‐Free Synthesized α‐Helical Transmembrane Protein NS4B from Hepatitis C Virus

Proton‐detected 100 kHz magic‐angle‐spinning (MAS) solid‐state NMR is an emerging analysis method for proteins with only hundreds of microgram quantities, and thus allows structural investigation of eukaryotic membrane proteins. This is the case for the cell‐free synthesized hepatitis C virus (HCV)...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jirasko, Vlastimil, Lakomek, Nils‐Alexander, Penzel, Susanne, Fogeron, Marie‐Laure, Bartenschlager, Ralf, Meier, Beat H., Böckmann, Anja
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2020
Materias:	Full Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318649/ https://www.ncbi.nlm.nih.gov/pubmed/31850615 http://dx.doi.org/10.1002/cbic.201900765

Ejemplares similares

Dimer Organization of Membrane‐Associated NS5A of Hepatitis C Virus as Determined by Highly Sensitive (1)H‐Detected Solid‐State NMR
por: Jirasko, Vlastimil, et al.
Publicado: (2021)

Solid-State NMR for Studying the Structure and Dynamics of Viral Assemblies
por: Lecoq, Lauriane, et al.
Publicado: (2020)

Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation
por: Wang, Shishan, et al.
Publicado: (2019)

A Conserved NS3 Surface Patch Orchestrates NS2 Protease Stimulation, NS5A Hyperphosphorylation and HCV Genome Replication
por: Isken, Olaf, et al.
Publicado: (2015)

Correction: A Conserved NS3 Surface Patch Orchestrates NS2 Protease Stimulation, NS5A Hyperphosphorylation and HCV Genome Replication
por: Isken, Olaf, et al.
Publicado: (2016)

100 kHz MAS Proton-Detected NMR Spectroscopy of Hepatitis B Virus Capsids
por: Lecoq, Lauriane, et al.
Publicado: (2019)

Spinning faster: protein NMR at MAS frequencies up to 126 kHz
por: Penzel, Susanne, et al.
Publicado: (2019)

Fast Magic‐Angle‐Spinning NMR Reveals the Evasive Hepatitis B Virus Capsid C‐Terminal Domain
por: Callon, Morgane, et al.
Publicado: (2022)

Temperature-Dependent Solid-State NMR Proton Chemical-Shift Values and Hydrogen Bonding
por: Malär, Alexander A., et al.
Publicado: (2021)

Including Protons in Solid-State NMR Resonance Assignment and Secondary Structure Analysis: The Example of RNA Polymerase II Subunits Rpo4/7
por: Torosyan, Anahit, et al.
Publicado: (2019)

Molecular elucidation of drug-induced abnormal assemblies of the hepatitis B virus capsid protein by solid-state NMR
por: Lecoq, Lauriane, et al.
Publicado: (2023)

High and fast: NMR protein–proton side-chain assignments at 160 kHz and 1.2 GHz
por: Callon, Morgane, et al.
Publicado: (2023)

Transmembrane α helices
por: Mall, Sanjay, et al.
Publicado: (2002)

Structure determination of the seven-helical transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy
por: Gautier, Antoine, et al.
Publicado: (2010)

Structural and Functional Studies of Nonstructural Protein 2 of the Hepatitis C Virus Reveal Its Key Role as Organizer of Virion Assembly
por: Jirasko, Vlastimil, et al.
Publicado: (2010)

Unlike Twins: An NMR Comparison of Two α-Synuclein Polymorphs Featuring Different Toxicity
por: Gath, Julia, et al.
Publicado: (2014)

Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology
por: Fogeron, Marie-Laure, et al.
Publicado: (2021)

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution
por: Callon, Morgane, et al.
Publicado: (2021)

Protein NMR Spectroscopy at 150 kHz Magic‐Angle Spinning Continues To Improve Resolution and Mass Sensitivity
por: Schledorn, Maarten, et al.
Publicado: (2020)

Folding and Insertion of Transmembrane Helices at the ER
por: Whitley, Paul, et al.
Publicado: (2021)

Chaperoning transmembrane helices in the lipid bilayer
por: Zhang, Qi, et al.
Publicado: (2020)

ER membrane protein complex is required for the insertions of late-synthesized transmembrane helices of Rh1 in Drosophila photoreceptors
por: Hiramatsu, Naoki, et al.
Publicado: (2019)

Asparagine and Glutamine Side-Chains and Ladders in HET-s(218–289) Amyloid Fibrils Studied by Fast Magic-Angle Spinning NMR
por: Wiegand, Thomas, et al.
Publicado: (2020)

Pharmacomodulation of a ligand targeting the HBV capsid hydrophobic pocket
por: Briday, Mathilde, et al.
Publicado: (2022)

Paramagnetic Solid‐State NMR to Localize the Metal‐Ion Cofactor in an Oligomeric DnaB Helicase
por: Zehnder, Johannes, et al.
Publicado: (2021)

Sedimentation Yields Long-Term Stable Protein Samples as Shown by Solid-State NMR
por: Wiegand, Thomas, et al.
Publicado: (2020)

Transmembrane helical interactions in the CFTR channel pore
por: Das, Jhuma, et al.
Publicado: (2017)

Solid-State NMR Reveals Asymmetric ATP Hydrolysis in the Multidrug ABC Transporter BmrA
por: Lacabanne, Denis, et al.
Publicado: (2022)

Solid-State NMR Provides Evidence for Small-Amplitude Slow Domain Motions in a Multispanning Transmembrane α-Helical Protein
por: Good, Daryl, et al.
Publicado: (2017)

Correction of field instabilities in biomolecular solid-state NMR by simultaneous acquisition of a frequency reference
por: Římal, Václav, et al.
Publicado: (2022)

Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies
por: Kunert, Britta, et al.
Publicado: (2014)

MemBrain: Improving the Accuracy of Predicting Transmembrane Helices
por: Shen, Hongbin, et al.
Publicado: (2008)

Formation of Transmembrane Helices In Vivo—Is Hydrophobicity All that Matters?
por: von Heijne, Gunnar
Publicado: (2007)

Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics
por: Olšinová, Marie, et al.
Publicado: (2018)

Transmembrane but not soluble helices fold inside the ribosome tunnel
por: Bañó-Polo, Manuel, et al.
Publicado: (2018)

Reassessment of MxiH subunit orientation and fold within native Shigella T3SS needles using surface labelling and solid-state NMR
por: Verasdonck, Joeri, et al.
Publicado: (2015)

Accessing ns–μs side chain dynamics in ubiquitin with methyl RDCs
por: Farès, Christophe, et al.
Publicado: (2009)

NS2 proteases from hepatitis C virus and related hepaciviruses share composite active sites and previously unrecognized intrinsic proteolytic activities
por: Boukadida, Célia, et al.
Publicado: (2018)

Nucleotide Binding Modes in a Motor Protein Revealed by (31)P‐ and (1)H‐Detected MAS Solid‐State NMR Spectroscopy
por: Wiegand, Thomas, et al.
Publicado: (2019)

Transmembrane Helices 7 and 8 Confer Aggregation Sensitivity to the Cystic Fibrosis Transmembrane Conductance Regulator
por: Kleizen, Bertrand, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_9ne5svh0f6m2h8jrh2c439eord