Cargando…

Human Fibroblasts as a Model for the Study of Bone Disorders

Bone tissue degeneration is an urgent clinical issue, making it a subject of intensive research. Chronic skeletal disease forms can be prevalent, such as the age-related osteoporosis, or rare, in the form of monogenetic bone disorders. A barrier in the understanding of the underlying pathological pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Claeys, Lauria, Bravenboer, Nathalie, Eekhoff, Elisabeth M. W., Micha, Dimitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318867/
https://www.ncbi.nlm.nih.gov/pubmed/32636804
http://dx.doi.org/10.3389/fendo.2020.00394
_version_ 1783550945513177088
author Claeys, Lauria
Bravenboer, Nathalie
Eekhoff, Elisabeth M. W.
Micha, Dimitra
author_facet Claeys, Lauria
Bravenboer, Nathalie
Eekhoff, Elisabeth M. W.
Micha, Dimitra
author_sort Claeys, Lauria
collection PubMed
description Bone tissue degeneration is an urgent clinical issue, making it a subject of intensive research. Chronic skeletal disease forms can be prevalent, such as the age-related osteoporosis, or rare, in the form of monogenetic bone disorders. A barrier in the understanding of the underlying pathological process is the lack of accessibility to relevant material. For this reason, cells of non-bone tissue are emerging as a suitable alternative for models of bone biology. Fibroblasts are highly suitable for this application; they populate accessible anatomical locations, such as the skin tissue. Reports suggesting their utility in preclinical models for the study of skeletal diseases are increasingly becoming available. The majority of these are based on the generation of an intermediate stem cell type, the induced pluripotent stem cells, which are subsequently directed to the osteogenic cell lineage. This intermediate stage is circumvented in transdifferentiation, the process regulating the direct conversion of fibroblasts to osteogenic cells, which is currently not well-explored. With this mini review, we aimed to give an overview of existing osteogenic transdifferentiation models and to inform about their applications in bone biology models.
format Online
Article
Text
id pubmed-7318867
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-73188672020-07-06 Human Fibroblasts as a Model for the Study of Bone Disorders Claeys, Lauria Bravenboer, Nathalie Eekhoff, Elisabeth M. W. Micha, Dimitra Front Endocrinol (Lausanne) Endocrinology Bone tissue degeneration is an urgent clinical issue, making it a subject of intensive research. Chronic skeletal disease forms can be prevalent, such as the age-related osteoporosis, or rare, in the form of monogenetic bone disorders. A barrier in the understanding of the underlying pathological process is the lack of accessibility to relevant material. For this reason, cells of non-bone tissue are emerging as a suitable alternative for models of bone biology. Fibroblasts are highly suitable for this application; they populate accessible anatomical locations, such as the skin tissue. Reports suggesting their utility in preclinical models for the study of skeletal diseases are increasingly becoming available. The majority of these are based on the generation of an intermediate stem cell type, the induced pluripotent stem cells, which are subsequently directed to the osteogenic cell lineage. This intermediate stage is circumvented in transdifferentiation, the process regulating the direct conversion of fibroblasts to osteogenic cells, which is currently not well-explored. With this mini review, we aimed to give an overview of existing osteogenic transdifferentiation models and to inform about their applications in bone biology models. Frontiers Media S.A. 2020-06-19 /pmc/articles/PMC7318867/ /pubmed/32636804 http://dx.doi.org/10.3389/fendo.2020.00394 Text en Copyright © 2020 Claeys, Bravenboer, Eekhoff and Micha. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Claeys, Lauria
Bravenboer, Nathalie
Eekhoff, Elisabeth M. W.
Micha, Dimitra
Human Fibroblasts as a Model for the Study of Bone Disorders
title Human Fibroblasts as a Model for the Study of Bone Disorders
title_full Human Fibroblasts as a Model for the Study of Bone Disorders
title_fullStr Human Fibroblasts as a Model for the Study of Bone Disorders
title_full_unstemmed Human Fibroblasts as a Model for the Study of Bone Disorders
title_short Human Fibroblasts as a Model for the Study of Bone Disorders
title_sort human fibroblasts as a model for the study of bone disorders
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318867/
https://www.ncbi.nlm.nih.gov/pubmed/32636804
http://dx.doi.org/10.3389/fendo.2020.00394
work_keys_str_mv AT claeyslauria humanfibroblastsasamodelforthestudyofbonedisorders
AT bravenboernathalie humanfibroblastsasamodelforthestudyofbonedisorders
AT eekhoffelisabethmw humanfibroblastsasamodelforthestudyofbonedisorders
AT michadimitra humanfibroblastsasamodelforthestudyofbonedisorders