Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification

Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit – the 2019 Novel Coronavirus Detection Kit (nCoV-DK) – halves the detection time by elimi...

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Detalles Bibliográficos
Autores principales: Fukumoto, Tatsuya, Iwasaki, Sumio, Fujisawa, Shinichi, Hayasaka, Kasumi, Sato, Kaori, Oguri, Satoshi, Taki, Keisuke, Nakakubo, Sho, Kamada, Keisuke, Yamashita, Yu, Konno, Satoshi, Nishida, Mutsumi, Sugita, Junichi, Teshima, Takanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318955/
https://www.ncbi.nlm.nih.gov/pubmed/32599282
http://dx.doi.org/10.1016/j.ijid.2020.06.074
Descripción
Sumario:Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit – the 2019 Novel Coronavirus Detection Kit (nCoV-DK) – halves the detection time by eliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error.

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