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Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses

To unravel the virome in birch trees of German and Finnish origin exhibiting symptoms of birch leaf-roll disease (BRLD), high-throughput sequencing (HTS) was employed. In total five viruses, among which three were so far unknown, were detected by RNAseq. One to five virus variants were identified in...

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Autores principales: Rumbou, Artemis, Candresse, Thierry, Marais, Armelle, Svanella-Dumas, Laurence, Landgraf, Maria, von Bargen, Susanne, Büttner, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319284/
https://www.ncbi.nlm.nih.gov/pubmed/32589631
http://dx.doi.org/10.1371/journal.pone.0221834
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author Rumbou, Artemis
Candresse, Thierry
Marais, Armelle
Svanella-Dumas, Laurence
Landgraf, Maria
von Bargen, Susanne
Büttner, Carmen
author_facet Rumbou, Artemis
Candresse, Thierry
Marais, Armelle
Svanella-Dumas, Laurence
Landgraf, Maria
von Bargen, Susanne
Büttner, Carmen
author_sort Rumbou, Artemis
collection PubMed
description To unravel the virome in birch trees of German and Finnish origin exhibiting symptoms of birch leaf-roll disease (BRLD), high-throughput sequencing (HTS) was employed. In total five viruses, among which three were so far unknown, were detected by RNAseq. One to five virus variants were identified in the transcriptome of individual trees. The novel viruses were genetically—fully or partially—characterized, belonging to the genera Carlavirus, Idaeovirus and Capillovirus and are tentatively named birch carlavirus, birch idaeovirus, and birch capillovirus, respectively. The recently discovered birch leafroll-associated virus was systematically detected by HTS in symptomatic seedlings but not in symptomless ones. The new carlavirus was detected only in one of the three symptomatic seedlings. The novel putative Capillovirus was detected in all seedlings—irrespective of their BLRD status—while the Idaeovirus was identified in a plant without leaf symptoms at the time of sampling. Further efforts are needed to complete Koch’s postulates and to clarify the possible association of the detected viruses with the BLR disease. Our study elucidates the viral population in single birch seedlings and provides a comprehensive overview for the diversities of the viral communities they harbor, to date.
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spelling pubmed-73192842020-06-30 Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses Rumbou, Artemis Candresse, Thierry Marais, Armelle Svanella-Dumas, Laurence Landgraf, Maria von Bargen, Susanne Büttner, Carmen PLoS One Research Article To unravel the virome in birch trees of German and Finnish origin exhibiting symptoms of birch leaf-roll disease (BRLD), high-throughput sequencing (HTS) was employed. In total five viruses, among which three were so far unknown, were detected by RNAseq. One to five virus variants were identified in the transcriptome of individual trees. The novel viruses were genetically—fully or partially—characterized, belonging to the genera Carlavirus, Idaeovirus and Capillovirus and are tentatively named birch carlavirus, birch idaeovirus, and birch capillovirus, respectively. The recently discovered birch leafroll-associated virus was systematically detected by HTS in symptomatic seedlings but not in symptomless ones. The new carlavirus was detected only in one of the three symptomatic seedlings. The novel putative Capillovirus was detected in all seedlings—irrespective of their BLRD status—while the Idaeovirus was identified in a plant without leaf symptoms at the time of sampling. Further efforts are needed to complete Koch’s postulates and to clarify the possible association of the detected viruses with the BLR disease. Our study elucidates the viral population in single birch seedlings and provides a comprehensive overview for the diversities of the viral communities they harbor, to date. Public Library of Science 2020-06-26 /pmc/articles/PMC7319284/ /pubmed/32589631 http://dx.doi.org/10.1371/journal.pone.0221834 Text en © 2020 Rumbou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rumbou, Artemis
Candresse, Thierry
Marais, Armelle
Svanella-Dumas, Laurence
Landgraf, Maria
von Bargen, Susanne
Büttner, Carmen
Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses
title Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses
title_full Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses
title_fullStr Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses
title_full_unstemmed Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses
title_short Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses
title_sort unravelling the virome in birch: rna-seq reveals a complex of known and novel viruses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319284/
https://www.ncbi.nlm.nih.gov/pubmed/32589631
http://dx.doi.org/10.1371/journal.pone.0221834
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