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Activated whole-body arginine pathway in high-active mice

Our previous studies suggest that physical activity (PA) levels are potentially regulated by endogenous metabolic mechanisms such as the vasodilatory roles of nitric oxide (NO) production via the precursor arginine (ARG) and ARG-related pathways. We assessed ARG metabolism and its precursors [citrul...

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Autores principales: Granados, Jorge Z., Ten Have, Gabriella A. M., Letsinger, Ayland C., Thaden, John J., Engelen, Marielle P. K. J., Lightfoot, J. Timothy, Deutz, Nicolaas E. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319332/
https://www.ncbi.nlm.nih.gov/pubmed/32589680
http://dx.doi.org/10.1371/journal.pone.0235095
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author Granados, Jorge Z.
Ten Have, Gabriella A. M.
Letsinger, Ayland C.
Thaden, John J.
Engelen, Marielle P. K. J.
Lightfoot, J. Timothy
Deutz, Nicolaas E. P.
author_facet Granados, Jorge Z.
Ten Have, Gabriella A. M.
Letsinger, Ayland C.
Thaden, John J.
Engelen, Marielle P. K. J.
Lightfoot, J. Timothy
Deutz, Nicolaas E. P.
author_sort Granados, Jorge Z.
collection PubMed
description Our previous studies suggest that physical activity (PA) levels are potentially regulated by endogenous metabolic mechanisms such as the vasodilatory roles of nitric oxide (NO) production via the precursor arginine (ARG) and ARG-related pathways. We assessed ARG metabolism and its precursors [citrulline (CIT), glutamine (GLN), glutamate (GLU), ornithine (ORN), and phenylalanine (PHE)] by measuring plasma concentration, whole-body production (WBP), de novo ARG and NO production, and clearance rates in previously classified low-active (LA) or high-active (HA) mice. We assessed LA (n = 23) and HA (n = 20) male mice by administering a stable isotope tracer pulse via jugular catheterization. We measured plasma enrichments via liquid chromatography tandem mass spectrometry (LC-MS/MS) and body compostion by echo-MRI. WBP, clearance rates, and de novo ARG and NO were calculated. Compared to LA mice, HA mice had lower plasma concentrations of GLU (71.1%; 36.8 ± 2.9 vs. 17.5 ± 1.7μM; p<0.0001), CIT (21%; 57.3 ± 2.3 vs. 46.4 ± 1.5μM; p = 0.0003), and ORN (40.1%; 55.4 ± 7.3 vs. 36.9 ± 2.6μM; p = 0.0241), but no differences for GLN, PHE, and ARG. However, HA mice had higher estimated NO production ratio (0.64 ± 0.08; p = 0.0197), higher WBP for CIT (21.8%, 8.6 ± 0.2 vs. 10.7 ± 0.3 nmol/g-lbm/min; p<0.0001), ARG (21.4%, 35.0 ± 0.6 vs. 43.4 ± 0.7 nmol/g-lbm/min; p<0.0001), PHE (7.6%, 23.8 ± 0.5 vs. 25.6 ± 0.5 nmol/g-lbm/min; p<0.0100), and lower GLU (78.5%; 9.4 ± 1.1 vs. 4.1 ± 1.6 nmol/g lbm/min; p = 0.0161). We observed no significant differences in WBP for GLN, ORN, PHE, or de novo ARG. We concluded that HA mice have an activated whole-body ARG pathway, which may be associated with regulating PA levels via increased NO production.
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spelling pubmed-73193322020-06-30 Activated whole-body arginine pathway in high-active mice Granados, Jorge Z. Ten Have, Gabriella A. M. Letsinger, Ayland C. Thaden, John J. Engelen, Marielle P. K. J. Lightfoot, J. Timothy Deutz, Nicolaas E. P. PLoS One Research Article Our previous studies suggest that physical activity (PA) levels are potentially regulated by endogenous metabolic mechanisms such as the vasodilatory roles of nitric oxide (NO) production via the precursor arginine (ARG) and ARG-related pathways. We assessed ARG metabolism and its precursors [citrulline (CIT), glutamine (GLN), glutamate (GLU), ornithine (ORN), and phenylalanine (PHE)] by measuring plasma concentration, whole-body production (WBP), de novo ARG and NO production, and clearance rates in previously classified low-active (LA) or high-active (HA) mice. We assessed LA (n = 23) and HA (n = 20) male mice by administering a stable isotope tracer pulse via jugular catheterization. We measured plasma enrichments via liquid chromatography tandem mass spectrometry (LC-MS/MS) and body compostion by echo-MRI. WBP, clearance rates, and de novo ARG and NO were calculated. Compared to LA mice, HA mice had lower plasma concentrations of GLU (71.1%; 36.8 ± 2.9 vs. 17.5 ± 1.7μM; p<0.0001), CIT (21%; 57.3 ± 2.3 vs. 46.4 ± 1.5μM; p = 0.0003), and ORN (40.1%; 55.4 ± 7.3 vs. 36.9 ± 2.6μM; p = 0.0241), but no differences for GLN, PHE, and ARG. However, HA mice had higher estimated NO production ratio (0.64 ± 0.08; p = 0.0197), higher WBP for CIT (21.8%, 8.6 ± 0.2 vs. 10.7 ± 0.3 nmol/g-lbm/min; p<0.0001), ARG (21.4%, 35.0 ± 0.6 vs. 43.4 ± 0.7 nmol/g-lbm/min; p<0.0001), PHE (7.6%, 23.8 ± 0.5 vs. 25.6 ± 0.5 nmol/g-lbm/min; p<0.0100), and lower GLU (78.5%; 9.4 ± 1.1 vs. 4.1 ± 1.6 nmol/g lbm/min; p = 0.0161). We observed no significant differences in WBP for GLN, ORN, PHE, or de novo ARG. We concluded that HA mice have an activated whole-body ARG pathway, which may be associated with regulating PA levels via increased NO production. Public Library of Science 2020-06-26 /pmc/articles/PMC7319332/ /pubmed/32589680 http://dx.doi.org/10.1371/journal.pone.0235095 Text en © 2020 Granados et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Granados, Jorge Z.
Ten Have, Gabriella A. M.
Letsinger, Ayland C.
Thaden, John J.
Engelen, Marielle P. K. J.
Lightfoot, J. Timothy
Deutz, Nicolaas E. P.
Activated whole-body arginine pathway in high-active mice
title Activated whole-body arginine pathway in high-active mice
title_full Activated whole-body arginine pathway in high-active mice
title_fullStr Activated whole-body arginine pathway in high-active mice
title_full_unstemmed Activated whole-body arginine pathway in high-active mice
title_short Activated whole-body arginine pathway in high-active mice
title_sort activated whole-body arginine pathway in high-active mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319332/
https://www.ncbi.nlm.nih.gov/pubmed/32589680
http://dx.doi.org/10.1371/journal.pone.0235095
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