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Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation
The detection of erythropoietin (Epo) protein by Western blotting has required pre‐purification of the sample. We developed a new Western blot method to detect plasma and urinary Epo using deglycosylation. Epo in urine and tissue, and erythropoiesis‐stimulating agents (ESAs) in urine were directly d...
Autores principales: | Yasuoka, Yukiko, Fukuyama, Takashi, Izumi, Yuichiro, Nakayama, Yushi, Inoue, Hideki, Yanagita, Kengo, Oshima, Tomomi, Yamazaki, Taiga, Uematsu, Takayuki, Kobayashi, Noritada, Shimada, Yoshitaka, Nagaba, Yasushi, Mukoyama, Masashi, Yamashita, Tetsuro, Sato, Yuichi, Sands, Jeff M., Kawahara, Katsumasa, Nonoguchi, Hiroshi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319944/ https://www.ncbi.nlm.nih.gov/pubmed/32592328 http://dx.doi.org/10.14814/phy2.14485 |
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