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CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells
The development of metastases is the major cause of cancer related death. To develop a standardized method that define the ability of human cancer cells to degrade the basement membrane, e.g. the delamination capacity, is of importance to assess metastatic aggressiveness. We now present the in vivo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320147/ https://www.ncbi.nlm.nih.gov/pubmed/32591581 http://dx.doi.org/10.1038/s41598-020-67492-7 |
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author | Palaniappan, Tamilarasan K. Šlekienė, Lina Jonasson, Anna-Karin Gilthorpe, Jonathan Gunhaga, Lena |
author_facet | Palaniappan, Tamilarasan K. Šlekienė, Lina Jonasson, Anna-Karin Gilthorpe, Jonathan Gunhaga, Lena |
author_sort | Palaniappan, Tamilarasan K. |
collection | PubMed |
description | The development of metastases is the major cause of cancer related death. To develop a standardized method that define the ability of human cancer cells to degrade the basement membrane, e.g. the delamination capacity, is of importance to assess metastatic aggressiveness. We now present the in vivo CAM-Delam assay to visualize and quantify the ability of human cancer cells to delaminate and invade. The method includes seeding cancer cells on the chick chorioallantoic membrane (CAM), followed by the evaluation of cancer-induced delamination and potential invasion within hours to a few days. By testing a range of human cancer cell lines in the CAM-Delam assay, our results show that the delamination capacity can be divided into four categories and used to quantify metastatic aggressiveness. Our results emphasize the usefulness of this assay for quantifying delamination capacity as a measurement of metastatic aggressiveness, and in unraveling the molecular mechanisms that regulate delamination, invasion, formation of micro-metastases and modulations of the tumor microenvironment. This method will be useful in both the preclinical and clinical characterization of tumor biopsies, and in the validation of compounds that may improve survival in metastatic cancer. |
format | Online Article Text |
id | pubmed-7320147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73201472020-06-30 CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells Palaniappan, Tamilarasan K. Šlekienė, Lina Jonasson, Anna-Karin Gilthorpe, Jonathan Gunhaga, Lena Sci Rep Article The development of metastases is the major cause of cancer related death. To develop a standardized method that define the ability of human cancer cells to degrade the basement membrane, e.g. the delamination capacity, is of importance to assess metastatic aggressiveness. We now present the in vivo CAM-Delam assay to visualize and quantify the ability of human cancer cells to delaminate and invade. The method includes seeding cancer cells on the chick chorioallantoic membrane (CAM), followed by the evaluation of cancer-induced delamination and potential invasion within hours to a few days. By testing a range of human cancer cell lines in the CAM-Delam assay, our results show that the delamination capacity can be divided into four categories and used to quantify metastatic aggressiveness. Our results emphasize the usefulness of this assay for quantifying delamination capacity as a measurement of metastatic aggressiveness, and in unraveling the molecular mechanisms that regulate delamination, invasion, formation of micro-metastases and modulations of the tumor microenvironment. This method will be useful in both the preclinical and clinical characterization of tumor biopsies, and in the validation of compounds that may improve survival in metastatic cancer. Nature Publishing Group UK 2020-06-26 /pmc/articles/PMC7320147/ /pubmed/32591581 http://dx.doi.org/10.1038/s41598-020-67492-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Palaniappan, Tamilarasan K. Šlekienė, Lina Jonasson, Anna-Karin Gilthorpe, Jonathan Gunhaga, Lena CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells |
title | CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells |
title_full | CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells |
title_fullStr | CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells |
title_full_unstemmed | CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells |
title_short | CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells |
title_sort | cam-delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320147/ https://www.ncbi.nlm.nih.gov/pubmed/32591581 http://dx.doi.org/10.1038/s41598-020-67492-7 |
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