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An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins

The biological clock of cyanobacteria is composed of three proteins, KaiA, KaiB, and KaiC. The KaiB-KaiC binding brings the slowness into the system, which is essential for the long period of the circadian rhythm. However, there is no consensus as to the origin of the slowness due to the pre-binding...

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Autores principales: Koda, Shin-ichi, Saito, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320175/
https://www.ncbi.nlm.nih.gov/pubmed/32591637
http://dx.doi.org/10.1038/s41598-020-67298-7
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author Koda, Shin-ichi
Saito, Shinji
author_facet Koda, Shin-ichi
Saito, Shinji
author_sort Koda, Shin-ichi
collection PubMed
description The biological clock of cyanobacteria is composed of three proteins, KaiA, KaiB, and KaiC. The KaiB-KaiC binding brings the slowness into the system, which is essential for the long period of the circadian rhythm. However, there is no consensus as to the origin of the slowness due to the pre-binding conformational transition of either KaiB or KaiC. In this study, we propose a simple KaiB-KaiC binding scheme in a hexameric form with an attractive interaction between adjacent bound KaiB monomers, which is independent of KaiB’s conformational change. We then show that the present scheme can explain several important experimental results on the binding, including that used as evidence for the slow conformational transition of KaiB. The present result thus indicates that the slowness arises from KaiC rather than KaiB.
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spelling pubmed-73201752020-06-30 An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins Koda, Shin-ichi Saito, Shinji Sci Rep Article The biological clock of cyanobacteria is composed of three proteins, KaiA, KaiB, and KaiC. The KaiB-KaiC binding brings the slowness into the system, which is essential for the long period of the circadian rhythm. However, there is no consensus as to the origin of the slowness due to the pre-binding conformational transition of either KaiB or KaiC. In this study, we propose a simple KaiB-KaiC binding scheme in a hexameric form with an attractive interaction between adjacent bound KaiB monomers, which is independent of KaiB’s conformational change. We then show that the present scheme can explain several important experimental results on the binding, including that used as evidence for the slow conformational transition of KaiB. The present result thus indicates that the slowness arises from KaiC rather than KaiB. Nature Publishing Group UK 2020-06-26 /pmc/articles/PMC7320175/ /pubmed/32591637 http://dx.doi.org/10.1038/s41598-020-67298-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Koda, Shin-ichi
Saito, Shinji
An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins
title An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins
title_full An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins
title_fullStr An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins
title_full_unstemmed An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins
title_short An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins
title_sort alternative interpretation of the slow kaib-kaic binding of the cyanobacterial clock proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320175/
https://www.ncbi.nlm.nih.gov/pubmed/32591637
http://dx.doi.org/10.1038/s41598-020-67298-7
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