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Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort

A renaissance in cell-free protein synthesis (CFPS) is underway, enabled by the acceleration and adoption of synthetic biology methods. CFPS has emerged as a powerful platform technology for synthetic gene network design, biosensing and on-demand biomanufacturing. Whilst primarily of bacterial origi...

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Detalles Bibliográficos
Autores principales: Spice, Alex J., Aw, Rochelle, Bracewell, Daniel G., Polizzi, Karen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320237/
https://www.ncbi.nlm.nih.gov/pubmed/32637667
http://dx.doi.org/10.1016/j.synbio.2020.06.003