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Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics

During the contact lens (CL) usage, microbial adhesion and biofilm formation are crucial threats for eye health due to the development of mature biofilms on CL surfaces associated with serious eye infections such as keratitis. For CL related eye infections, multi drug resistant Pseudomonas aeruginos...

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Autores principales: Dosler, Sibel, Hacioglu, Mayram, Yilmaz, Fatima Nur, Oyardi, Ozlem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320721/
https://www.ncbi.nlm.nih.gov/pubmed/32612893
http://dx.doi.org/10.7717/peerj.9419
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author Dosler, Sibel
Hacioglu, Mayram
Yilmaz, Fatima Nur
Oyardi, Ozlem
author_facet Dosler, Sibel
Hacioglu, Mayram
Yilmaz, Fatima Nur
Oyardi, Ozlem
author_sort Dosler, Sibel
collection PubMed
description During the contact lens (CL) usage, microbial adhesion and biofilm formation are crucial threats for eye health due to the development of mature biofilms on CL surfaces associated with serious eye infections such as keratitis. For CL related eye infections, multi drug resistant Pseudomonas aeruginosa or Staphylococcus aureus (especially MRSA) and Candida albicans are the most common infectious bacteria and yeast, respectively. In this study, CL biofilm models were created by comparing them to reveal the differences on specific conditions. Then the anti-biofilm activities of some commercially available multipurpose CL solutions (MPSs) and antibiotic eye drops against mature biofilms of S. aureus, P. aeruginosa, and C. albicans standard and clinical strains were determined by the time killing curve (TKC) method at 6, 24 and 48 h. According to the biofilm formation models, the optimal biofilms occurred in a mixture of bovine serum albumin (20% v/v) and lysozyme (2 g/L) diluted in PBS at 37 °C for 24 h, without shaking. When we compared the CL types under the same conditions, the strongest biofilms according to their cell density, were formed on Pure Vision ≥ Softens 38 > Acuve 2 ∼ Softens Toric CLs. When we compared the used CLs with the new ones, a significant increase at the density of biofilms on the used CLs was observed. The most active MPS against P. aeruginosa and S. aureus biofilms at 24 h was Opti-Free followed by Bio-True and Renu according to the TKC analyses. In addition, the most active MPS against C. albicans was Renu followed by Opti-Free and Bio-True at 48 h. None of the MPSs showed 3 Log bactericidal/fungicidal activity, except for Opti-Free against S. aureus and P. aeruginosa biofilms during 6 h contact time. Moreover, all studied antibiotic eye drops were active against S. aureus and P. aeruginosa biofilms on CLs at 6 h and 24 h either directly or as 1/10 concentration, respectively. According to the results of the study, anti-biofilm activities of MPSs have changed depending on the chemical ingredients and contact times of MPSs, the type of infectious agent, and especially the CL type and usage time.
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spelling pubmed-73207212020-06-30 Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics Dosler, Sibel Hacioglu, Mayram Yilmaz, Fatima Nur Oyardi, Ozlem PeerJ Microbiology During the contact lens (CL) usage, microbial adhesion and biofilm formation are crucial threats for eye health due to the development of mature biofilms on CL surfaces associated with serious eye infections such as keratitis. For CL related eye infections, multi drug resistant Pseudomonas aeruginosa or Staphylococcus aureus (especially MRSA) and Candida albicans are the most common infectious bacteria and yeast, respectively. In this study, CL biofilm models were created by comparing them to reveal the differences on specific conditions. Then the anti-biofilm activities of some commercially available multipurpose CL solutions (MPSs) and antibiotic eye drops against mature biofilms of S. aureus, P. aeruginosa, and C. albicans standard and clinical strains were determined by the time killing curve (TKC) method at 6, 24 and 48 h. According to the biofilm formation models, the optimal biofilms occurred in a mixture of bovine serum albumin (20% v/v) and lysozyme (2 g/L) diluted in PBS at 37 °C for 24 h, without shaking. When we compared the CL types under the same conditions, the strongest biofilms according to their cell density, were formed on Pure Vision ≥ Softens 38 > Acuve 2 ∼ Softens Toric CLs. When we compared the used CLs with the new ones, a significant increase at the density of biofilms on the used CLs was observed. The most active MPS against P. aeruginosa and S. aureus biofilms at 24 h was Opti-Free followed by Bio-True and Renu according to the TKC analyses. In addition, the most active MPS against C. albicans was Renu followed by Opti-Free and Bio-True at 48 h. None of the MPSs showed 3 Log bactericidal/fungicidal activity, except for Opti-Free against S. aureus and P. aeruginosa biofilms during 6 h contact time. Moreover, all studied antibiotic eye drops were active against S. aureus and P. aeruginosa biofilms on CLs at 6 h and 24 h either directly or as 1/10 concentration, respectively. According to the results of the study, anti-biofilm activities of MPSs have changed depending on the chemical ingredients and contact times of MPSs, the type of infectious agent, and especially the CL type and usage time. PeerJ Inc. 2020-06-24 /pmc/articles/PMC7320721/ /pubmed/32612893 http://dx.doi.org/10.7717/peerj.9419 Text en ©2020 Dosler et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Dosler, Sibel
Hacioglu, Mayram
Yilmaz, Fatima Nur
Oyardi, Ozlem
Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics
title Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics
title_full Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics
title_fullStr Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics
title_full_unstemmed Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics
title_short Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics
title_sort biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320721/
https://www.ncbi.nlm.nih.gov/pubmed/32612893
http://dx.doi.org/10.7717/peerj.9419
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