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Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane
Due to the increasing use of antibiotics, measures are being taken to improve their removal from the natural environment. The support of biodegradation with natural surfactants that increase the bioavailability of impurities for microorganisms that degrade them, raises questions about their effect o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321062/ https://www.ncbi.nlm.nih.gov/pubmed/32481761 http://dx.doi.org/10.3390/molecules25112527 |
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author | Rojewska, Monika Smułek, Wojciech Prochaska, Krystyna Kaczorek, Ewa |
author_facet | Rojewska, Monika Smułek, Wojciech Prochaska, Krystyna Kaczorek, Ewa |
author_sort | Rojewska, Monika |
collection | PubMed |
description | Due to the increasing use of antibiotics, measures are being taken to improve their removal from the natural environment. The support of biodegradation with natural surfactants that increase the bioavailability of impurities for microorganisms that degrade them, raises questions about their effect on bacterial cells. In this paper we present analysis of the interaction of nitrofurantoin (NFT) and saponins from the Saponaria officinalis on the environmental bacteria membrane and the model phospholipid membrane mimicking it. A wide perspective of the process is provided with the Langmuir monolayer technique and membrane permeability test with bacteria. The obtained results showed that above critical micelle concentration (CMC), saponin molecules are incorporated into the POPE monolayer, but the NFT impact was ambiguous. What is more, differences in membrane permeability between the cells exposed to NFT in comparison to that of the non-exposed cells were observed above 1.0 CMC for Achromobacter sp. KW1 or above 0.5 CMC for Pseudomonas sp. MChB. In both cases, NFT presence lowered the membrane permeability. Moreover, the Congo red adhesion to the cell membrane also decreased in the presence of a high concentration of surfactants and NFT. The results suggest that saponins are incorporated into the bacteria membrane, but their sugar hydrophilic part remains outside, which modifies the adsorption properties of the cell surface as well as the membrane permeability. |
format | Online Article Text |
id | pubmed-7321062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73210622020-07-06 Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane Rojewska, Monika Smułek, Wojciech Prochaska, Krystyna Kaczorek, Ewa Molecules Article Due to the increasing use of antibiotics, measures are being taken to improve their removal from the natural environment. The support of biodegradation with natural surfactants that increase the bioavailability of impurities for microorganisms that degrade them, raises questions about their effect on bacterial cells. In this paper we present analysis of the interaction of nitrofurantoin (NFT) and saponins from the Saponaria officinalis on the environmental bacteria membrane and the model phospholipid membrane mimicking it. A wide perspective of the process is provided with the Langmuir monolayer technique and membrane permeability test with bacteria. The obtained results showed that above critical micelle concentration (CMC), saponin molecules are incorporated into the POPE monolayer, but the NFT impact was ambiguous. What is more, differences in membrane permeability between the cells exposed to NFT in comparison to that of the non-exposed cells were observed above 1.0 CMC for Achromobacter sp. KW1 or above 0.5 CMC for Pseudomonas sp. MChB. In both cases, NFT presence lowered the membrane permeability. Moreover, the Congo red adhesion to the cell membrane also decreased in the presence of a high concentration of surfactants and NFT. The results suggest that saponins are incorporated into the bacteria membrane, but their sugar hydrophilic part remains outside, which modifies the adsorption properties of the cell surface as well as the membrane permeability. MDPI 2020-05-28 /pmc/articles/PMC7321062/ /pubmed/32481761 http://dx.doi.org/10.3390/molecules25112527 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rojewska, Monika Smułek, Wojciech Prochaska, Krystyna Kaczorek, Ewa Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane |
title | Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane |
title_full | Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane |
title_fullStr | Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane |
title_full_unstemmed | Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane |
title_short | Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane |
title_sort | combined effect of nitrofurantoin and plant surfactant on bacteria phospholipid membrane |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321062/ https://www.ncbi.nlm.nih.gov/pubmed/32481761 http://dx.doi.org/10.3390/molecules25112527 |
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