Cargando…

Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine

The highly contagious Newcastle disease virus (NDV) continues to threaten poultry all over the world. The NDV DNA vaccine is a promising solution to the current Newcastle disease (ND) challenges, and thus an efficient delivery system should be developed to facilitate the efficacy of DNA vaccines. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Ying, Ji, Hui, Peng, Lin, Gao, Xiuge, Jiang, Shanxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321080/
https://www.ncbi.nlm.nih.gov/pubmed/32481518
http://dx.doi.org/10.3390/molecules25112505
_version_ 1783551382097231872
author Gao, Ying
Ji, Hui
Peng, Lin
Gao, Xiuge
Jiang, Shanxiang
author_facet Gao, Ying
Ji, Hui
Peng, Lin
Gao, Xiuge
Jiang, Shanxiang
author_sort Gao, Ying
collection PubMed
description The highly contagious Newcastle disease virus (NDV) continues to threaten poultry all over the world. The NDV DNA vaccine is a promising solution to the current Newcastle disease (ND) challenges, and thus an efficient delivery system should be developed to facilitate the efficacy of DNA vaccines. In this study, we developed a DNA vaccine delivery system consisting of a triblock copolymer of poly(lactide co-glycolide acid) and polyethylene glycol (PLGA-PEG-PLGA) hydrogel in which the recombinant NDV hemagglutinin-neuraminidase (HN) plasmid was encapsulated. Its characteristics, security, immune responses, and efficacy against highly virulent NDV were detected. The results showed that the plasmids were gradually released in a sustained manner from the hydrogel, which improved the biological stability of the plasmids and demonstrated a high biocompatibility. The plasmids, when they were incorporated into the hydrogel delivery system, enhanced immune activation and provided 100% protection against the highly virulent NDV strain. Furthermore, we proved that this NDV DNA hydrogel vaccine could improve the lymphocyte proliferation and increase the immunological cytokine production via the PI3K/Akt pathway. These results indicate that the PLGA-PEG-PLGA thermosensitive hydrogel could be a promising delivery system for the NDV DNA vaccine in order to achieve a sustained supply of plasmids and induce potent immune responses.
format Online
Article
Text
id pubmed-7321080
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73210802020-07-06 Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine Gao, Ying Ji, Hui Peng, Lin Gao, Xiuge Jiang, Shanxiang Molecules Article The highly contagious Newcastle disease virus (NDV) continues to threaten poultry all over the world. The NDV DNA vaccine is a promising solution to the current Newcastle disease (ND) challenges, and thus an efficient delivery system should be developed to facilitate the efficacy of DNA vaccines. In this study, we developed a DNA vaccine delivery system consisting of a triblock copolymer of poly(lactide co-glycolide acid) and polyethylene glycol (PLGA-PEG-PLGA) hydrogel in which the recombinant NDV hemagglutinin-neuraminidase (HN) plasmid was encapsulated. Its characteristics, security, immune responses, and efficacy against highly virulent NDV were detected. The results showed that the plasmids were gradually released in a sustained manner from the hydrogel, which improved the biological stability of the plasmids and demonstrated a high biocompatibility. The plasmids, when they were incorporated into the hydrogel delivery system, enhanced immune activation and provided 100% protection against the highly virulent NDV strain. Furthermore, we proved that this NDV DNA hydrogel vaccine could improve the lymphocyte proliferation and increase the immunological cytokine production via the PI3K/Akt pathway. These results indicate that the PLGA-PEG-PLGA thermosensitive hydrogel could be a promising delivery system for the NDV DNA vaccine in order to achieve a sustained supply of plasmids and induce potent immune responses. MDPI 2020-05-28 /pmc/articles/PMC7321080/ /pubmed/32481518 http://dx.doi.org/10.3390/molecules25112505 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gao, Ying
Ji, Hui
Peng, Lin
Gao, Xiuge
Jiang, Shanxiang
Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine
title Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine
title_full Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine
title_fullStr Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine
title_full_unstemmed Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine
title_short Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine
title_sort development of plga-peg-plga hydrogel delivery system for enhanced immunoreaction and efficacy of newcastle disease virus dna vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321080/
https://www.ncbi.nlm.nih.gov/pubmed/32481518
http://dx.doi.org/10.3390/molecules25112505
work_keys_str_mv AT gaoying developmentofplgapegplgahydrogeldeliverysystemforenhancedimmunoreactionandefficacyofnewcastlediseasevirusdnavaccine
AT jihui developmentofplgapegplgahydrogeldeliverysystemforenhancedimmunoreactionandefficacyofnewcastlediseasevirusdnavaccine
AT penglin developmentofplgapegplgahydrogeldeliverysystemforenhancedimmunoreactionandefficacyofnewcastlediseasevirusdnavaccine
AT gaoxiuge developmentofplgapegplgahydrogeldeliverysystemforenhancedimmunoreactionandefficacyofnewcastlediseasevirusdnavaccine
AT jiangshanxiang developmentofplgapegplgahydrogeldeliverysystemforenhancedimmunoreactionandefficacyofnewcastlediseasevirusdnavaccine