Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts

A simple and fast high-performance liquid chromatography (HPLC) fingerprint method combining reference standard extract for the identification of bilberry extract was developed and validated. Six batches of bilberry extract collected from different manufactures were used to establish the HPLC finger...

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Detalles Bibliográficos
Autores principales: Liu, Bingbing, Hu, Tiantian, Yan, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321295/
https://www.ncbi.nlm.nih.gov/pubmed/32481617
http://dx.doi.org/10.3390/molecules25112514
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author Liu, Bingbing
Hu, Tiantian
Yan, Weidong
author_facet Liu, Bingbing
Hu, Tiantian
Yan, Weidong
author_sort Liu, Bingbing
collection PubMed
description A simple and fast high-performance liquid chromatography (HPLC) fingerprint method combining reference standard extract for the identification of bilberry extract was developed and validated. Six batches of bilberry extract collected from different manufactures were used to establish the HPLC fingerprint. Other berry extracts—such as blueberry extracts, mulberry extracts, cranberry extracts, and black rice extracts—were also analyzed for their HPLC chromatograms. The fingerprints of five batches of bilberry extract showed high similarities, while one batch was distinguished from others. Additionally, the content of anthocyanin Cyanidin-3-O-glucoside (Cy-3-glc) in each berry extract was analyzed and compared. The results indicate that this HPLC fingerprint method, combining reference standard extracts, could be used for the authentication and quality control of bilberry extracts.
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spelling pubmed-73212952020-06-29 Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts Liu, Bingbing Hu, Tiantian Yan, Weidong Molecules Article A simple and fast high-performance liquid chromatography (HPLC) fingerprint method combining reference standard extract for the identification of bilberry extract was developed and validated. Six batches of bilberry extract collected from different manufactures were used to establish the HPLC fingerprint. Other berry extracts—such as blueberry extracts, mulberry extracts, cranberry extracts, and black rice extracts—were also analyzed for their HPLC chromatograms. The fingerprints of five batches of bilberry extract showed high similarities, while one batch was distinguished from others. Additionally, the content of anthocyanin Cyanidin-3-O-glucoside (Cy-3-glc) in each berry extract was analyzed and compared. The results indicate that this HPLC fingerprint method, combining reference standard extracts, could be used for the authentication and quality control of bilberry extracts. MDPI 2020-05-28 /pmc/articles/PMC7321295/ /pubmed/32481617 http://dx.doi.org/10.3390/molecules25112514 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Bingbing
Hu, Tiantian
Yan, Weidong
Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts
title Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts
title_full Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts
title_fullStr Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts
title_full_unstemmed Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts
title_short Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts
title_sort authentication of the bilberry extracts by an hplc fingerprint method combining reference standard extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321295/
https://www.ncbi.nlm.nih.gov/pubmed/32481617
http://dx.doi.org/10.3390/molecules25112514
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AT yanweidong authenticationofthebilberryextractsbyanhplcfingerprintmethodcombiningreferencestandardextracts

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