Cargando…

Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds

Glycerol and alkanediols are being studied as alternative solvents to extract phytochemicals from plant material, often as hydrogen bond donors in deep eutectic solvents (DESs). Many of those alcohols are liquid at room temperature, yet studies of their use as extraction solvents are scarce. In this...

Descripción completa

Detalles Bibliográficos
Autores principales: Vieira, Vanessa, Calhelha, Ricardo C., Barros, Lillian, Coutinho, João A. P., C. F. R. Ferreira, Isabel, Ferreira, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321402/
https://www.ncbi.nlm.nih.gov/pubmed/32471297
http://dx.doi.org/10.3390/molecules25112497
_version_ 1783551457368211456
author Vieira, Vanessa
Calhelha, Ricardo C.
Barros, Lillian
Coutinho, João A. P.
C. F. R. Ferreira, Isabel
Ferreira, Olga
author_facet Vieira, Vanessa
Calhelha, Ricardo C.
Barros, Lillian
Coutinho, João A. P.
C. F. R. Ferreira, Isabel
Ferreira, Olga
author_sort Vieira, Vanessa
collection PubMed
description Glycerol and alkanediols are being studied as alternative solvents to extract phytochemicals from plant material, often as hydrogen bond donors in deep eutectic solvents (DESs). Many of those alcohols are liquid at room temperature, yet studies of their use as extraction solvents are scarce. In this work, glycerol and a series of alkanediols (1,2-ethanediol, 1,2-propanediol, 1,3-propanediol, 1,3-butanediol, 1,2-pentanediol, 1,5-pentanediol, and 1,2-hexanediol) were studied for the extraction of phenolic compounds from Juglans regia L. leaves, a rich source of this class of bioactive compounds. The extraction yield was quantified, and the bioactivity of both extracts and pure solvents was evaluated by measuring the anti-inflammatory and cytotoxic activities. The solvents showing the best combined results were 1,2 and 1,3-propanediol, as their extracts presented a high amount of phenolic compounds, close to the results of ethanol, and similar cytotoxicity against cervical carcinoma cells, with no impact on non-tumor porcine liver cells in the studied concentration range. On the other hand, none of the extracts (and solvents) presented anti-inflammatory activity. Overall, the results obtained in this work contribute to the study of alternative solvents that could potentially be used also as formulation media, highlighting the importance of walnut leaves as a source of bioactive compounds.
format Online
Article
Text
id pubmed-7321402
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73214022020-06-29 Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds Vieira, Vanessa Calhelha, Ricardo C. Barros, Lillian Coutinho, João A. P. C. F. R. Ferreira, Isabel Ferreira, Olga Molecules Article Glycerol and alkanediols are being studied as alternative solvents to extract phytochemicals from plant material, often as hydrogen bond donors in deep eutectic solvents (DESs). Many of those alcohols are liquid at room temperature, yet studies of their use as extraction solvents are scarce. In this work, glycerol and a series of alkanediols (1,2-ethanediol, 1,2-propanediol, 1,3-propanediol, 1,3-butanediol, 1,2-pentanediol, 1,5-pentanediol, and 1,2-hexanediol) were studied for the extraction of phenolic compounds from Juglans regia L. leaves, a rich source of this class of bioactive compounds. The extraction yield was quantified, and the bioactivity of both extracts and pure solvents was evaluated by measuring the anti-inflammatory and cytotoxic activities. The solvents showing the best combined results were 1,2 and 1,3-propanediol, as their extracts presented a high amount of phenolic compounds, close to the results of ethanol, and similar cytotoxicity against cervical carcinoma cells, with no impact on non-tumor porcine liver cells in the studied concentration range. On the other hand, none of the extracts (and solvents) presented anti-inflammatory activity. Overall, the results obtained in this work contribute to the study of alternative solvents that could potentially be used also as formulation media, highlighting the importance of walnut leaves as a source of bioactive compounds. MDPI 2020-05-27 /pmc/articles/PMC7321402/ /pubmed/32471297 http://dx.doi.org/10.3390/molecules25112497 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vieira, Vanessa
Calhelha, Ricardo C.
Barros, Lillian
Coutinho, João A. P.
C. F. R. Ferreira, Isabel
Ferreira, Olga
Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds
title Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds
title_full Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds
title_fullStr Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds
title_full_unstemmed Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds
title_short Insights on the Extraction Performance of Alkanediols and Glycerol: Using Juglans regia L. Leaves as a Source of Bioactive Compounds
title_sort insights on the extraction performance of alkanediols and glycerol: using juglans regia l. leaves as a source of bioactive compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321402/
https://www.ncbi.nlm.nih.gov/pubmed/32471297
http://dx.doi.org/10.3390/molecules25112497
work_keys_str_mv AT vieiravanessa insightsontheextractionperformanceofalkanediolsandglycerolusingjuglansregialleavesasasourceofbioactivecompounds
AT calhelharicardoc insightsontheextractionperformanceofalkanediolsandglycerolusingjuglansregialleavesasasourceofbioactivecompounds
AT barroslillian insightsontheextractionperformanceofalkanediolsandglycerolusingjuglansregialleavesasasourceofbioactivecompounds
AT coutinhojoaoap insightsontheextractionperformanceofalkanediolsandglycerolusingjuglansregialleavesasasourceofbioactivecompounds
AT cfrferreiraisabel insightsontheextractionperformanceofalkanediolsandglycerolusingjuglansregialleavesasasourceofbioactivecompounds
AT ferreiraolga insightsontheextractionperformanceofalkanediolsandglycerolusingjuglansregialleavesasasourceofbioactivecompounds