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Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect

The main objective of this research is to demonstrate the biocompatibility of two experimental graphene dental materials by in vitro and in vivo tests for applications in dentistry. The novel graphene dental materials, including one restorative composite and one dental cement, were subjected to cyto...

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Autores principales: Dreanca, Alexandra, Sarosi, Codruta, Parvu, Alina Elena, Blidaru, Mihai, Enacrachi, George, Purdoiu, Robert, Nagy, Andras, Sevastre, Bogdan, Oros, Nechita Adrian, Marcus, Ioan, Moldovan, Marioara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321491/
https://www.ncbi.nlm.nih.gov/pubmed/32486437
http://dx.doi.org/10.3390/ma13112511
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author Dreanca, Alexandra
Sarosi, Codruta
Parvu, Alina Elena
Blidaru, Mihai
Enacrachi, George
Purdoiu, Robert
Nagy, Andras
Sevastre, Bogdan
Oros, Nechita Adrian
Marcus, Ioan
Moldovan, Marioara
author_facet Dreanca, Alexandra
Sarosi, Codruta
Parvu, Alina Elena
Blidaru, Mihai
Enacrachi, George
Purdoiu, Robert
Nagy, Andras
Sevastre, Bogdan
Oros, Nechita Adrian
Marcus, Ioan
Moldovan, Marioara
author_sort Dreanca, Alexandra
collection PubMed
description The main objective of this research is to demonstrate the biocompatibility of two experimental graphene dental materials by in vitro and in vivo tests for applications in dentistry. The novel graphene dental materials, including one restorative composite and one dental cement, were subjected to cytotoxicity and implantation tests by using a rat model of a non-critical mandibular defect. In vitro cytotoxicity induced by materials on human dental follicle stem cells (restorative composite) and dysplastic oral keratinocytes (dental cement) was investigated at 37 °C for 24 h. After in vivo implantation, at 7 weeks, bone samples were harvested and subjected to histological investigations. The plasma biochemistry, oxidative stress, and sub-chronic organ toxicity analysis were also performed. The resulting cytotoxicity tests confirm that the materials had no toxic effects against dental cells after 24 h. Following graphene dental materials implantation, the animals did not present any symptoms of acute toxicity or local inflammation. No alterations were detected in relative organ weights and in correlation with hepatic and renal histological findings. The materials’ lack of systemic organ toxicity was confirmed. The outcomes of our study provided further evidence on the graphene dental materials’ ability for bone regeneration and biocompatibility.
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spelling pubmed-73214912020-06-29 Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect Dreanca, Alexandra Sarosi, Codruta Parvu, Alina Elena Blidaru, Mihai Enacrachi, George Purdoiu, Robert Nagy, Andras Sevastre, Bogdan Oros, Nechita Adrian Marcus, Ioan Moldovan, Marioara Materials (Basel) Article The main objective of this research is to demonstrate the biocompatibility of two experimental graphene dental materials by in vitro and in vivo tests for applications in dentistry. The novel graphene dental materials, including one restorative composite and one dental cement, were subjected to cytotoxicity and implantation tests by using a rat model of a non-critical mandibular defect. In vitro cytotoxicity induced by materials on human dental follicle stem cells (restorative composite) and dysplastic oral keratinocytes (dental cement) was investigated at 37 °C for 24 h. After in vivo implantation, at 7 weeks, bone samples were harvested and subjected to histological investigations. The plasma biochemistry, oxidative stress, and sub-chronic organ toxicity analysis were also performed. The resulting cytotoxicity tests confirm that the materials had no toxic effects against dental cells after 24 h. Following graphene dental materials implantation, the animals did not present any symptoms of acute toxicity or local inflammation. No alterations were detected in relative organ weights and in correlation with hepatic and renal histological findings. The materials’ lack of systemic organ toxicity was confirmed. The outcomes of our study provided further evidence on the graphene dental materials’ ability for bone regeneration and biocompatibility. MDPI 2020-05-31 /pmc/articles/PMC7321491/ /pubmed/32486437 http://dx.doi.org/10.3390/ma13112511 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dreanca, Alexandra
Sarosi, Codruta
Parvu, Alina Elena
Blidaru, Mihai
Enacrachi, George
Purdoiu, Robert
Nagy, Andras
Sevastre, Bogdan
Oros, Nechita Adrian
Marcus, Ioan
Moldovan, Marioara
Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect
title Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect
title_full Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect
title_fullStr Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect
title_full_unstemmed Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect
title_short Systemic and Local Biocompatibility Assessment of Graphene Composite Dental Materials in Experimental Mandibular Bone Defect
title_sort systemic and local biocompatibility assessment of graphene composite dental materials in experimental mandibular bone defect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321491/
https://www.ncbi.nlm.nih.gov/pubmed/32486437
http://dx.doi.org/10.3390/ma13112511
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