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qPCR multiplex detection of microRNA and messenger RNA in a single reaction

Reverse Transcription-Quantitative PCR (RT-qPCR) is one of the standards for analytical measurement of different RNA species in biological models. However, current Reverse Transcription (RT) based priming strategies are unable to synthesize differing RNAs and ncRNAs especially miRNAs, within a singl...

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Detalles Bibliográficos
Autores principales: Khoury, Samantha, Tran, Nham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321665/
https://www.ncbi.nlm.nih.gov/pubmed/32617186
http://dx.doi.org/10.7717/peerj.9004