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Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Intestinal microbiota perform important functions for the health of fishes. Knowing the microbial composition and evaluating the possible effects caused by anthropogenic pollution in the intestinal microbiota of fish populations might represent an important step in defining microbial biomarkers for...

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Autores principales: Nolorbe-Payahua, Christian Deyvis, de Freitas, Anderson Santos, Roesch, Luiz Fernando Wurdig, Zanette, Juliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322053/
https://www.ncbi.nlm.nih.gov/pubmed/32613104
http://dx.doi.org/10.1016/j.heliyon.2020.e04190
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author Nolorbe-Payahua, Christian Deyvis
de Freitas, Anderson Santos
Roesch, Luiz Fernando Wurdig
Zanette, Juliano
author_facet Nolorbe-Payahua, Christian Deyvis
de Freitas, Anderson Santos
Roesch, Luiz Fernando Wurdig
Zanette, Juliano
author_sort Nolorbe-Payahua, Christian Deyvis
collection PubMed
description Intestinal microbiota perform important functions for the health of fishes. Knowing the microbial composition and evaluating the possible effects caused by anthropogenic pollution in the intestinal microbiota of fish populations might represent an important step in defining microbial biomarkers for water pollution. This study evaluated the impact of environmental contamination on the gut microbiota of the livebearer killifish Phalloceros caudimaculatus. The 16S survey using the V4 region of the 16S rRNA gene was used to characterize and compare the microbiota of two P. caudimaculatus populations from streams with different levels of environmental contamination in Rio Grande, RS, Brazil. Twelve bacterial operational taxonomic units (OTUs) (around one-third of the total) were shared between both fish populations. They represent the core microbiota of the gut in this species. The dominant phyla were Protebacteria and Firmicutes, with more than 80% of relative abundance. The dominant genus was Burkholderia with more than 35% of the relative abundance irrespective of the environmental condition. We detected a lower microbial diversity (Shannon index and observed OTUs) in fish from the polluted stream compared to the reference stream. The PERMANOVA analysis showed that the intestinal microbial communities from fish living in the polluted stream were distinct from those found in the reference stream (p < 0.05). Finally, we identified Luteolibacter, Methylocaldum and Rhodobacter genera, which correlated strongly with the polluted stream. These taxa might represent potential microbial biomarkers of exposure to environmental contaminants in the guts of fish. Confirmation of these findings in other polluted environments might allow the development of a microbiota-based screening approach for environmental evaluation in ecotoxicological studies in aquatic ecosystems.
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spelling pubmed-73220532020-06-30 Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus Nolorbe-Payahua, Christian Deyvis de Freitas, Anderson Santos Roesch, Luiz Fernando Wurdig Zanette, Juliano Heliyon Article Intestinal microbiota perform important functions for the health of fishes. Knowing the microbial composition and evaluating the possible effects caused by anthropogenic pollution in the intestinal microbiota of fish populations might represent an important step in defining microbial biomarkers for water pollution. This study evaluated the impact of environmental contamination on the gut microbiota of the livebearer killifish Phalloceros caudimaculatus. The 16S survey using the V4 region of the 16S rRNA gene was used to characterize and compare the microbiota of two P. caudimaculatus populations from streams with different levels of environmental contamination in Rio Grande, RS, Brazil. Twelve bacterial operational taxonomic units (OTUs) (around one-third of the total) were shared between both fish populations. They represent the core microbiota of the gut in this species. The dominant phyla were Protebacteria and Firmicutes, with more than 80% of relative abundance. The dominant genus was Burkholderia with more than 35% of the relative abundance irrespective of the environmental condition. We detected a lower microbial diversity (Shannon index and observed OTUs) in fish from the polluted stream compared to the reference stream. The PERMANOVA analysis showed that the intestinal microbial communities from fish living in the polluted stream were distinct from those found in the reference stream (p < 0.05). Finally, we identified Luteolibacter, Methylocaldum and Rhodobacter genera, which correlated strongly with the polluted stream. These taxa might represent potential microbial biomarkers of exposure to environmental contaminants in the guts of fish. Confirmation of these findings in other polluted environments might allow the development of a microbiota-based screening approach for environmental evaluation in ecotoxicological studies in aquatic ecosystems. Elsevier 2020-06-23 /pmc/articles/PMC7322053/ /pubmed/32613104 http://dx.doi.org/10.1016/j.heliyon.2020.e04190 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Nolorbe-Payahua, Christian Deyvis
de Freitas, Anderson Santos
Roesch, Luiz Fernando Wurdig
Zanette, Juliano
Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus
title Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus
title_full Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus
title_fullStr Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus
title_full_unstemmed Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus
title_short Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus
title_sort environmental contamination alters the intestinal microbial community of the livebearer killifish phalloceros caudimaculatus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322053/
https://www.ncbi.nlm.nih.gov/pubmed/32613104
http://dx.doi.org/10.1016/j.heliyon.2020.e04190
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