Cargando…

Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias

Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the...

Descripción completa

Detalles Bibliográficos
Autores principales: Mashima, Kiyomi, Azuma, Morio, Fujiwara, Ken, Inagaki, Takashi, Oh, Iekuni, Ikeda, Takashi, Umino, Kento, Nakano, Hirofumi, Morita, Kaoru, Sato, Kazuya, Minakata, Daisuke, Yamasaki, Ryoko, Ashizawa, Masahiro, Yamamoto, Chihiro, Fujiwara, Shin-Ichiro, Hatano, Kaoru, Ohmine, Ken, Muroi, Kazuo, Ohno, Nobuhiko, Kanda, Yoshinobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322163/
https://www.ncbi.nlm.nih.gov/pubmed/32624629
http://dx.doi.org/10.1267/ahc.19035
_version_ 1783551589864177664
author Mashima, Kiyomi
Azuma, Morio
Fujiwara, Ken
Inagaki, Takashi
Oh, Iekuni
Ikeda, Takashi
Umino, Kento
Nakano, Hirofumi
Morita, Kaoru
Sato, Kazuya
Minakata, Daisuke
Yamasaki, Ryoko
Ashizawa, Masahiro
Yamamoto, Chihiro
Fujiwara, Shin-Ichiro
Hatano, Kaoru
Ohmine, Ken
Muroi, Kazuo
Ohno, Nobuhiko
Kanda, Yoshinobu
author_facet Mashima, Kiyomi
Azuma, Morio
Fujiwara, Ken
Inagaki, Takashi
Oh, Iekuni
Ikeda, Takashi
Umino, Kento
Nakano, Hirofumi
Morita, Kaoru
Sato, Kazuya
Minakata, Daisuke
Yamasaki, Ryoko
Ashizawa, Masahiro
Yamamoto, Chihiro
Fujiwara, Shin-Ichiro
Hatano, Kaoru
Ohmine, Ken
Muroi, Kazuo
Ohno, Nobuhiko
Kanda, Yoshinobu
author_sort Mashima, Kiyomi
collection PubMed
description Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the localization of tumor cells in the affected organs are still unclear. In this study, we histologically examined the distribution of leukemia cells in several organs using two leukemic mouse models produced by the administration of two cell lines (THP-1, a human myelomonocytic leukemia, and A20, a mouse B cell leukemia/lymphoma) to severe immunodeficient mice. Survival of the mice depended on the tumor burden. Although A20 and THP-1 tumor cells massively infiltrated the parenchyma of the liver and spleen at 21 days after transplantation, A20 cells were hardly found in connective tissues in Glisson’s capsule in the liver as compared with THP-1 cells. In the bone marrow, there was more severe infiltration of A20 cells than THP-1 cells. THP-1 and A20 cells were widely spread in the lungs, but were rarely observed in the small intestine. These findings suggest that each leukemia model has a unique localization of tumor cells in several affected organs, which could critically affect the disease course and the efficacy of therapeutic agents, including cellular immunotherapies.
format Online
Article
Text
id pubmed-7322163
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
record_format MEDLINE/PubMed
spelling pubmed-73221632020-07-02 Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias Mashima, Kiyomi Azuma, Morio Fujiwara, Ken Inagaki, Takashi Oh, Iekuni Ikeda, Takashi Umino, Kento Nakano, Hirofumi Morita, Kaoru Sato, Kazuya Minakata, Daisuke Yamasaki, Ryoko Ashizawa, Masahiro Yamamoto, Chihiro Fujiwara, Shin-Ichiro Hatano, Kaoru Ohmine, Ken Muroi, Kazuo Ohno, Nobuhiko Kanda, Yoshinobu Acta Histochem Cytochem Regular Article Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the localization of tumor cells in the affected organs are still unclear. In this study, we histologically examined the distribution of leukemia cells in several organs using two leukemic mouse models produced by the administration of two cell lines (THP-1, a human myelomonocytic leukemia, and A20, a mouse B cell leukemia/lymphoma) to severe immunodeficient mice. Survival of the mice depended on the tumor burden. Although A20 and THP-1 tumor cells massively infiltrated the parenchyma of the liver and spleen at 21 days after transplantation, A20 cells were hardly found in connective tissues in Glisson’s capsule in the liver as compared with THP-1 cells. In the bone marrow, there was more severe infiltration of A20 cells than THP-1 cells. THP-1 and A20 cells were widely spread in the lungs, but were rarely observed in the small intestine. These findings suggest that each leukemia model has a unique localization of tumor cells in several affected organs, which could critically affect the disease course and the efficacy of therapeutic agents, including cellular immunotherapies. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2020-06-26 2020-04-29 /pmc/articles/PMC7322163/ /pubmed/32624629 http://dx.doi.org/10.1267/ahc.19035 Text en 2020 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Mashima, Kiyomi
Azuma, Morio
Fujiwara, Ken
Inagaki, Takashi
Oh, Iekuni
Ikeda, Takashi
Umino, Kento
Nakano, Hirofumi
Morita, Kaoru
Sato, Kazuya
Minakata, Daisuke
Yamasaki, Ryoko
Ashizawa, Masahiro
Yamamoto, Chihiro
Fujiwara, Shin-Ichiro
Hatano, Kaoru
Ohmine, Ken
Muroi, Kazuo
Ohno, Nobuhiko
Kanda, Yoshinobu
Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias
title Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias
title_full Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias
title_fullStr Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias
title_full_unstemmed Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias
title_short Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias
title_sort differential localization and invasion of tumor cells in mouse models of human and murine leukemias
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322163/
https://www.ncbi.nlm.nih.gov/pubmed/32624629
http://dx.doi.org/10.1267/ahc.19035
work_keys_str_mv AT mashimakiyomi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT azumamorio differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT fujiwaraken differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT inagakitakashi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT ohiekuni differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT ikedatakashi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT uminokento differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT nakanohirofumi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT moritakaoru differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT satokazuya differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT minakatadaisuke differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT yamasakiryoko differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT ashizawamasahiro differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT yamamotochihiro differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT fujiwarashinichiro differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT hatanokaoru differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT ohmineken differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT muroikazuo differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT ohnonobuhiko differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias
AT kandayoshinobu differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias