Cargando…
Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias
Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322163/ https://www.ncbi.nlm.nih.gov/pubmed/32624629 http://dx.doi.org/10.1267/ahc.19035 |
_version_ | 1783551589864177664 |
---|---|
author | Mashima, Kiyomi Azuma, Morio Fujiwara, Ken Inagaki, Takashi Oh, Iekuni Ikeda, Takashi Umino, Kento Nakano, Hirofumi Morita, Kaoru Sato, Kazuya Minakata, Daisuke Yamasaki, Ryoko Ashizawa, Masahiro Yamamoto, Chihiro Fujiwara, Shin-Ichiro Hatano, Kaoru Ohmine, Ken Muroi, Kazuo Ohno, Nobuhiko Kanda, Yoshinobu |
author_facet | Mashima, Kiyomi Azuma, Morio Fujiwara, Ken Inagaki, Takashi Oh, Iekuni Ikeda, Takashi Umino, Kento Nakano, Hirofumi Morita, Kaoru Sato, Kazuya Minakata, Daisuke Yamasaki, Ryoko Ashizawa, Masahiro Yamamoto, Chihiro Fujiwara, Shin-Ichiro Hatano, Kaoru Ohmine, Ken Muroi, Kazuo Ohno, Nobuhiko Kanda, Yoshinobu |
author_sort | Mashima, Kiyomi |
collection | PubMed |
description | Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the localization of tumor cells in the affected organs are still unclear. In this study, we histologically examined the distribution of leukemia cells in several organs using two leukemic mouse models produced by the administration of two cell lines (THP-1, a human myelomonocytic leukemia, and A20, a mouse B cell leukemia/lymphoma) to severe immunodeficient mice. Survival of the mice depended on the tumor burden. Although A20 and THP-1 tumor cells massively infiltrated the parenchyma of the liver and spleen at 21 days after transplantation, A20 cells were hardly found in connective tissues in Glisson’s capsule in the liver as compared with THP-1 cells. In the bone marrow, there was more severe infiltration of A20 cells than THP-1 cells. THP-1 and A20 cells were widely spread in the lungs, but were rarely observed in the small intestine. These findings suggest that each leukemia model has a unique localization of tumor cells in several affected organs, which could critically affect the disease course and the efficacy of therapeutic agents, including cellular immunotherapies. |
format | Online Article Text |
id | pubmed-7322163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY |
record_format | MEDLINE/PubMed |
spelling | pubmed-73221632020-07-02 Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias Mashima, Kiyomi Azuma, Morio Fujiwara, Ken Inagaki, Takashi Oh, Iekuni Ikeda, Takashi Umino, Kento Nakano, Hirofumi Morita, Kaoru Sato, Kazuya Minakata, Daisuke Yamasaki, Ryoko Ashizawa, Masahiro Yamamoto, Chihiro Fujiwara, Shin-Ichiro Hatano, Kaoru Ohmine, Ken Muroi, Kazuo Ohno, Nobuhiko Kanda, Yoshinobu Acta Histochem Cytochem Regular Article Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the localization of tumor cells in the affected organs are still unclear. In this study, we histologically examined the distribution of leukemia cells in several organs using two leukemic mouse models produced by the administration of two cell lines (THP-1, a human myelomonocytic leukemia, and A20, a mouse B cell leukemia/lymphoma) to severe immunodeficient mice. Survival of the mice depended on the tumor burden. Although A20 and THP-1 tumor cells massively infiltrated the parenchyma of the liver and spleen at 21 days after transplantation, A20 cells were hardly found in connective tissues in Glisson’s capsule in the liver as compared with THP-1 cells. In the bone marrow, there was more severe infiltration of A20 cells than THP-1 cells. THP-1 and A20 cells were widely spread in the lungs, but were rarely observed in the small intestine. These findings suggest that each leukemia model has a unique localization of tumor cells in several affected organs, which could critically affect the disease course and the efficacy of therapeutic agents, including cellular immunotherapies. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2020-06-26 2020-04-29 /pmc/articles/PMC7322163/ /pubmed/32624629 http://dx.doi.org/10.1267/ahc.19035 Text en 2020 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Mashima, Kiyomi Azuma, Morio Fujiwara, Ken Inagaki, Takashi Oh, Iekuni Ikeda, Takashi Umino, Kento Nakano, Hirofumi Morita, Kaoru Sato, Kazuya Minakata, Daisuke Yamasaki, Ryoko Ashizawa, Masahiro Yamamoto, Chihiro Fujiwara, Shin-Ichiro Hatano, Kaoru Ohmine, Ken Muroi, Kazuo Ohno, Nobuhiko Kanda, Yoshinobu Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias |
title | Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias |
title_full | Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias |
title_fullStr | Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias |
title_full_unstemmed | Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias |
title_short | Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias |
title_sort | differential localization and invasion of tumor cells in mouse models of human and murine leukemias |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322163/ https://www.ncbi.nlm.nih.gov/pubmed/32624629 http://dx.doi.org/10.1267/ahc.19035 |
work_keys_str_mv | AT mashimakiyomi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT azumamorio differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT fujiwaraken differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT inagakitakashi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT ohiekuni differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT ikedatakashi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT uminokento differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT nakanohirofumi differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT moritakaoru differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT satokazuya differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT minakatadaisuke differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT yamasakiryoko differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT ashizawamasahiro differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT yamamotochihiro differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT fujiwarashinichiro differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT hatanokaoru differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT ohmineken differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT muroikazuo differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT ohnonobuhiko differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias AT kandayoshinobu differentiallocalizationandinvasionoftumorcellsinmousemodelsofhumanandmurineleukemias |