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Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease
We profiled adaptive immunity in COVID-19 patients with active infection or after recovery and created a repository of currently >14 million B and T cell receptor (BCR and TCR) sequences from the blood of these patients. The B cell response showed converging IGHV3-driven BCR clusters closely asso...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324317/ https://www.ncbi.nlm.nih.gov/pubmed/32668194 http://dx.doi.org/10.1016/j.immuni.2020.06.024 |
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author | Schultheiß, Christoph Paschold, Lisa Simnica, Donjete Mohme, Malte Willscher, Edith von Wenserski, Lisa Scholz, Rebekka Wieters, Imke Dahlke, Christine Tolosa, Eva Sedding, Daniel G. Ciesek, Sandra Addo, Marylyn Binder, Mascha |
author_facet | Schultheiß, Christoph Paschold, Lisa Simnica, Donjete Mohme, Malte Willscher, Edith von Wenserski, Lisa Scholz, Rebekka Wieters, Imke Dahlke, Christine Tolosa, Eva Sedding, Daniel G. Ciesek, Sandra Addo, Marylyn Binder, Mascha |
author_sort | Schultheiß, Christoph |
collection | PubMed |
description | We profiled adaptive immunity in COVID-19 patients with active infection or after recovery and created a repository of currently >14 million B and T cell receptor (BCR and TCR) sequences from the blood of these patients. The B cell response showed converging IGHV3-driven BCR clusters closely associated with SARS-CoV-2 antibodies. Clonality and skewing of TCR repertoires were associated with interferon type I and III responses, early CD4(+) and CD8(+) T cell activation, and counterregulation by the co-receptors BTLA, Tim-3, PD-1, TIGIT, and CD73. Tfh, Th17-like, and nonconventional (but not classical antiviral) Th1 cell polarizations were induced. SARS-CoV-2-specific T cell responses were driven by TCR clusters shared between patients with a characteristic trajectory of clonotypes and traceability over the disease course. Our data provide fundamental insight into adaptive immunity to SARS-CoV-2 with the actively updated repository providing a resource for the scientific community urgently needed to inform therapeutic concepts and vaccine development. |
format | Online Article Text |
id | pubmed-7324317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73243172020-06-30 Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease Schultheiß, Christoph Paschold, Lisa Simnica, Donjete Mohme, Malte Willscher, Edith von Wenserski, Lisa Scholz, Rebekka Wieters, Imke Dahlke, Christine Tolosa, Eva Sedding, Daniel G. Ciesek, Sandra Addo, Marylyn Binder, Mascha Immunity Article We profiled adaptive immunity in COVID-19 patients with active infection or after recovery and created a repository of currently >14 million B and T cell receptor (BCR and TCR) sequences from the blood of these patients. The B cell response showed converging IGHV3-driven BCR clusters closely associated with SARS-CoV-2 antibodies. Clonality and skewing of TCR repertoires were associated with interferon type I and III responses, early CD4(+) and CD8(+) T cell activation, and counterregulation by the co-receptors BTLA, Tim-3, PD-1, TIGIT, and CD73. Tfh, Th17-like, and nonconventional (but not classical antiviral) Th1 cell polarizations were induced. SARS-CoV-2-specific T cell responses were driven by TCR clusters shared between patients with a characteristic trajectory of clonotypes and traceability over the disease course. Our data provide fundamental insight into adaptive immunity to SARS-CoV-2 with the actively updated repository providing a resource for the scientific community urgently needed to inform therapeutic concepts and vaccine development. Elsevier Inc. 2020-08-18 2020-06-30 /pmc/articles/PMC7324317/ /pubmed/32668194 http://dx.doi.org/10.1016/j.immuni.2020.06.024 Text en © 2020 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Schultheiß, Christoph Paschold, Lisa Simnica, Donjete Mohme, Malte Willscher, Edith von Wenserski, Lisa Scholz, Rebekka Wieters, Imke Dahlke, Christine Tolosa, Eva Sedding, Daniel G. Ciesek, Sandra Addo, Marylyn Binder, Mascha Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease |
title | Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease |
title_full | Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease |
title_fullStr | Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease |
title_full_unstemmed | Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease |
title_short | Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease |
title_sort | next-generation sequencing of t and b cell receptor repertoires from covid-19 patients showed signatures associated with severity of disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324317/ https://www.ncbi.nlm.nih.gov/pubmed/32668194 http://dx.doi.org/10.1016/j.immuni.2020.06.024 |
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