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Thyroid-related hormones as potential markers of hypoxia/ischemia

This study aimed to investigate the usefulness of the thyroid-related hormones as markers of acute systemic hypoxia/ischemia to identify deaths caused by asphyxiation due to neck compression in human autopsy cases. The following deaths from pathophysiological conditions were examined: mechanical asp...

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Autores principales: Tani, Naoto, Ishikawa, Mayumi, Watanabe, Miho, Ikeda, Tomoya, Ishikawa, Takaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324426/
https://www.ncbi.nlm.nih.gov/pubmed/32146707
http://dx.doi.org/10.1007/s13577-020-00341-x
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author Tani, Naoto
Ishikawa, Mayumi
Watanabe, Miho
Ikeda, Tomoya
Ishikawa, Takaki
author_facet Tani, Naoto
Ishikawa, Mayumi
Watanabe, Miho
Ikeda, Tomoya
Ishikawa, Takaki
author_sort Tani, Naoto
collection PubMed
description This study aimed to investigate the usefulness of the thyroid-related hormones as markers of acute systemic hypoxia/ischemia to identify deaths caused by asphyxiation due to neck compression in human autopsy cases. The following deaths from pathophysiological conditions were examined: mechanical asphyxia and acute/subacute blunt head injury; acute/subacute non-head blunt injury; sharp instrument injury as the hemorrhagic shock condition; drowning as alveolar injury; burn; and death due to cardiac dysfunction. Blood samples were collected from the left and right cardiac chambers and iliac veins, and serum triiodothyronine (T3), thyroxine (T4), thyroglobulin (Tg), and thyroid-stimulating hormone (TSH) levels were measured using electrochemiluminescence immunoassays. Two types of thyroid cell lines were used to confirm independent thyroid function under the condition of hypoxia (3% O(2)). The human thyroid carcinoma cell line (HOTHC) cell line derived from human anaplastic thyroid carcinoma and the UD-PTC (sample of the second resection papillary thyroid carcinoma) cell line derived from human thyroid papillary adenoma, which forms Tg retention follicles, were used to examine the secretion levels of T3, T4, and Tg hormones. The results showed a strong correlation between T3 and T4 levels in all blood sampling sites, while the TSH and Tg levels were not correlated with the other markers. Serum T3 and T4 levels were higher in cases of mechanical asphyxia and acute/subacute blunt head injury, representing hypoxic and ischemic conditions of the brain as compared to those in other causes of death. In the thyroid gland cell line, T4, T3, and Tg levels were stimulated after exposure to hypoxia for 10–30 min. These findings suggest that systemic advanced hypoxia/ischemia may cause a rapid and TSH-independent release of T3 and T4 thyroid hormones in autopsy cases. These findings demonstrate that increased thyroid-related hormone (T3 and T4) levels in the pathophysiological field may indicate systemic hypoxia/ischemia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13577-020-00341-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-73244262020-07-07 Thyroid-related hormones as potential markers of hypoxia/ischemia Tani, Naoto Ishikawa, Mayumi Watanabe, Miho Ikeda, Tomoya Ishikawa, Takaki Hum Cell Research Article This study aimed to investigate the usefulness of the thyroid-related hormones as markers of acute systemic hypoxia/ischemia to identify deaths caused by asphyxiation due to neck compression in human autopsy cases. The following deaths from pathophysiological conditions were examined: mechanical asphyxia and acute/subacute blunt head injury; acute/subacute non-head blunt injury; sharp instrument injury as the hemorrhagic shock condition; drowning as alveolar injury; burn; and death due to cardiac dysfunction. Blood samples were collected from the left and right cardiac chambers and iliac veins, and serum triiodothyronine (T3), thyroxine (T4), thyroglobulin (Tg), and thyroid-stimulating hormone (TSH) levels were measured using electrochemiluminescence immunoassays. Two types of thyroid cell lines were used to confirm independent thyroid function under the condition of hypoxia (3% O(2)). The human thyroid carcinoma cell line (HOTHC) cell line derived from human anaplastic thyroid carcinoma and the UD-PTC (sample of the second resection papillary thyroid carcinoma) cell line derived from human thyroid papillary adenoma, which forms Tg retention follicles, were used to examine the secretion levels of T3, T4, and Tg hormones. The results showed a strong correlation between T3 and T4 levels in all blood sampling sites, while the TSH and Tg levels were not correlated with the other markers. Serum T3 and T4 levels were higher in cases of mechanical asphyxia and acute/subacute blunt head injury, representing hypoxic and ischemic conditions of the brain as compared to those in other causes of death. In the thyroid gland cell line, T4, T3, and Tg levels were stimulated after exposure to hypoxia for 10–30 min. These findings suggest that systemic advanced hypoxia/ischemia may cause a rapid and TSH-independent release of T3 and T4 thyroid hormones in autopsy cases. These findings demonstrate that increased thyroid-related hormone (T3 and T4) levels in the pathophysiological field may indicate systemic hypoxia/ischemia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13577-020-00341-x) contains supplementary material, which is available to authorized users. Springer Singapore 2020-03-07 2020 /pmc/articles/PMC7324426/ /pubmed/32146707 http://dx.doi.org/10.1007/s13577-020-00341-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Tani, Naoto
Ishikawa, Mayumi
Watanabe, Miho
Ikeda, Tomoya
Ishikawa, Takaki
Thyroid-related hormones as potential markers of hypoxia/ischemia
title Thyroid-related hormones as potential markers of hypoxia/ischemia
title_full Thyroid-related hormones as potential markers of hypoxia/ischemia
title_fullStr Thyroid-related hormones as potential markers of hypoxia/ischemia
title_full_unstemmed Thyroid-related hormones as potential markers of hypoxia/ischemia
title_short Thyroid-related hormones as potential markers of hypoxia/ischemia
title_sort thyroid-related hormones as potential markers of hypoxia/ischemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324426/
https://www.ncbi.nlm.nih.gov/pubmed/32146707
http://dx.doi.org/10.1007/s13577-020-00341-x
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