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4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
BACKGROUND: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified. METHODS: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and inves...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324454/ https://www.ncbi.nlm.nih.gov/pubmed/32642459 http://dx.doi.org/10.1186/s40902-020-00267-2 |
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author | Kim, Min-Keun Kim, Seong-Gon Lee, Suk Keun |
author_facet | Kim, Min-Keun Kim, Seong-Gon Lee, Suk Keun |
author_sort | Kim, Min-Keun |
collection | PubMed |
description | BACKGROUND: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified. METHODS: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera. RESULTS: Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization. CONCLUSION: Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing. |
format | Online Article Text |
id | pubmed-7324454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-73244542020-07-07 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells Kim, Min-Keun Kim, Seong-Gon Lee, Suk Keun Maxillofac Plast Reconstr Surg Research BACKGROUND: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified. METHODS: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera. RESULTS: Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization. CONCLUSION: Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing. Springer Singapore 2020-06-29 /pmc/articles/PMC7324454/ /pubmed/32642459 http://dx.doi.org/10.1186/s40902-020-00267-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Kim, Min-Keun Kim, Seong-Gon Lee, Suk Keun 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells |
title | 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells |
title_full | 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells |
title_fullStr | 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells |
title_full_unstemmed | 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells |
title_short | 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells |
title_sort | 4-hexylresorcinol induced angiogenesis potential in human endothelial cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324454/ https://www.ncbi.nlm.nih.gov/pubmed/32642459 http://dx.doi.org/10.1186/s40902-020-00267-2 |
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