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4-Hexylresorcinol induced angiogenesis potential in human endothelial cells

BACKGROUND: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified. METHODS: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and inves...

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Autores principales: Kim, Min-Keun, Kim, Seong-Gon, Lee, Suk Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324454/
https://www.ncbi.nlm.nih.gov/pubmed/32642459
http://dx.doi.org/10.1186/s40902-020-00267-2
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author Kim, Min-Keun
Kim, Seong-Gon
Lee, Suk Keun
author_facet Kim, Min-Keun
Kim, Seong-Gon
Lee, Suk Keun
author_sort Kim, Min-Keun
collection PubMed
description BACKGROUND: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified. METHODS: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera. RESULTS: Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization. CONCLUSION: Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing.
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spelling pubmed-73244542020-07-07 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells Kim, Min-Keun Kim, Seong-Gon Lee, Suk Keun Maxillofac Plast Reconstr Surg Research BACKGROUND: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified. METHODS: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera. RESULTS: Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization. CONCLUSION: Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing. Springer Singapore 2020-06-29 /pmc/articles/PMC7324454/ /pubmed/32642459 http://dx.doi.org/10.1186/s40902-020-00267-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research
Kim, Min-Keun
Kim, Seong-Gon
Lee, Suk Keun
4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
title 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
title_full 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
title_fullStr 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
title_full_unstemmed 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
title_short 4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
title_sort 4-hexylresorcinol induced angiogenesis potential in human endothelial cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324454/
https://www.ncbi.nlm.nih.gov/pubmed/32642459
http://dx.doi.org/10.1186/s40902-020-00267-2
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