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A Generic Procedure for the Isolation of pH- and Magnesium-Responsive Chicken scFvs for Downstream Purification of Human Antibodies

Affinity chromatography provides an excellent platform for protein purification, which is a key step in the large scale downstream processing of therapeutic monoclonal antibodies (Mabs). Protein A chromatography constitutes the gold standard for Mab purification. However, the required acidic conditi...

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Detalles Bibliográficos
Autores principales:	Hinz, Steffen C., Elter, Adrian, Rammo, Oliver, Schwämmle, Achim, Ali, Ataurehman, Zielonka, Stefan, Herget, Thomas, Kolmar, Harald
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324474/ https://www.ncbi.nlm.nih.gov/pubmed/32656201 http://dx.doi.org/10.3389/fbioe.2020.00688

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