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Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production
Furfural is a major toxic byproduct found in the hydrolysate of lignocellulosic biomass, which adversely interferes with the growth and ethanol fermentation of Saccharomyces cerevisiae. The current study was focused on the impact of cofactor availability derived intracellular redox perturbation on f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324476/ https://www.ncbi.nlm.nih.gov/pubmed/32656198 http://dx.doi.org/10.3389/fbioe.2020.00615 |
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author | Liu, Chen-Guang Li, Kai Li, Ke-Yi Sakdaronnarong, Chularat Mehmood, Muhammad Aamer Zhao, Xin-Qing Bai, Feng-Wu |
author_facet | Liu, Chen-Guang Li, Kai Li, Ke-Yi Sakdaronnarong, Chularat Mehmood, Muhammad Aamer Zhao, Xin-Qing Bai, Feng-Wu |
author_sort | Liu, Chen-Guang |
collection | PubMed |
description | Furfural is a major toxic byproduct found in the hydrolysate of lignocellulosic biomass, which adversely interferes with the growth and ethanol fermentation of Saccharomyces cerevisiae. The current study was focused on the impact of cofactor availability derived intracellular redox perturbation on furfural tolerance. Here, three strategies were employed in cofactor conversion in S. cerevisiae: (1) heterologous expression of NADH dehydrogenase (NDH) from E. coli which catalyzed the NADH to NAD(+) and increased the cellular sensitivity to furfural, (2) overexpression of GLR1, OYE2, ZWF1, and IDP1 genes responsible for the interconversion of NADPH and NADP(+), which enhanced the furfural tolerance, (3) expression of NAD(P)(+) transhydrogenase (PNTB) and NAD(+) kinase (POS5) which showed a little impact on furfural tolerance. Besides, a substantial redistribution of metabolic fluxes was also observed with the expression of cofactor-related genes. These results indicated that NADPH-based intracellular redox perturbation plays a key role in furfural tolerance, which suggested single-gene manipulation as an effective strategy for enhancing tolerance and subsequently achieving higher ethanol titer using lignocellulosic hydrolysate. |
format | Online Article Text |
id | pubmed-7324476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73244762020-07-10 Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production Liu, Chen-Guang Li, Kai Li, Ke-Yi Sakdaronnarong, Chularat Mehmood, Muhammad Aamer Zhao, Xin-Qing Bai, Feng-Wu Front Bioeng Biotechnol Bioengineering and Biotechnology Furfural is a major toxic byproduct found in the hydrolysate of lignocellulosic biomass, which adversely interferes with the growth and ethanol fermentation of Saccharomyces cerevisiae. The current study was focused on the impact of cofactor availability derived intracellular redox perturbation on furfural tolerance. Here, three strategies were employed in cofactor conversion in S. cerevisiae: (1) heterologous expression of NADH dehydrogenase (NDH) from E. coli which catalyzed the NADH to NAD(+) and increased the cellular sensitivity to furfural, (2) overexpression of GLR1, OYE2, ZWF1, and IDP1 genes responsible for the interconversion of NADPH and NADP(+), which enhanced the furfural tolerance, (3) expression of NAD(P)(+) transhydrogenase (PNTB) and NAD(+) kinase (POS5) which showed a little impact on furfural tolerance. Besides, a substantial redistribution of metabolic fluxes was also observed with the expression of cofactor-related genes. These results indicated that NADPH-based intracellular redox perturbation plays a key role in furfural tolerance, which suggested single-gene manipulation as an effective strategy for enhancing tolerance and subsequently achieving higher ethanol titer using lignocellulosic hydrolysate. Frontiers Media S.A. 2020-06-23 /pmc/articles/PMC7324476/ /pubmed/32656198 http://dx.doi.org/10.3389/fbioe.2020.00615 Text en Copyright © 2020 Liu, Li, Li, Sakdaronnarong, Mehmood, Zhao and Bai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Liu, Chen-Guang Li, Kai Li, Ke-Yi Sakdaronnarong, Chularat Mehmood, Muhammad Aamer Zhao, Xin-Qing Bai, Feng-Wu Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production |
title | Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production |
title_full | Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production |
title_fullStr | Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production |
title_full_unstemmed | Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production |
title_short | Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production |
title_sort | intracellular redox perturbation in saccharomyces cerevisiae improved furfural tolerance and enhanced cellulosic bioethanol production |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324476/ https://www.ncbi.nlm.nih.gov/pubmed/32656198 http://dx.doi.org/10.3389/fbioe.2020.00615 |
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